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Troponin I Type 3 (cardiac) Antibody (679021) [Unconjugated]

 
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Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB68871) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Troponin I/TNNI3 at approximately 24-29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB68871) followed by HRP-conjugated Anti-Mouse IgG Secondary ...read more
Troponin I/TNNI3 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB68871) at 15 µg/mL overnight at 4 °C. Tissue ...read more
Simple Western lane view shows lysates of human heart tissue and human heart (ventricular) tissue, loaded at 0.5 mg/mL. Specific bands were detected for Troponin I at approximately 27-31 kDa (as indicated) using 10 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clone
679021
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB68871-SP
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Troponin I Type 3 (cardiac) Antibody (679021) [Unconjugated] Summary

Immunogen
Human cardiac Troponin I/TNNI3 purified from human heart
Specificity
Detects human cardiac Troponin I/TNNI3 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNNI3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Troponin I Type 3 (cardiac) Antibody (679021) [Unconjugated]

  • Cardiac troponin I
  • CMD1FF
  • CMD2A
  • CMH7MGC116817
  • CTnI
  • familial hypertrophic cardiomyopathy 7
  • RCM1
  • TNNC1
  • TNNC1troponin I, cardiac
  • TNNI3,
  • troponin I type 3 (cardiac)
  • troponin I, cardiac muscle
  • Troponin I/TNNI3

Background

Troponin I, also known as TNI, is a 24-29 kDa component of a protein complex on striated muscle thin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2 (encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with heriditary cardiomyopathy. Human cardiac Troponin I shares 93% amino acid sequence identity with mouse and rat cardiac Troponin I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Troponin I Type 3 (cardiac) Antibody (679021) [Unconjugated]
Species: Hu
Applications: WB, Simple Western, IHC

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Secondary Antibodies

 

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Additional Troponin I Type 3 (cardiac) Products

Blogs on Troponin I Type 3 (cardiac).

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Troponin I Type 3 (TnI, cardiac)
Troponin I is part of a heteromeric thin filament regulatory complex that governs skeletal and cardiac muscle contraction. Troponin I is composed of three subunits: troponin I (TnI), troponin T (TnT), and troponin C (TnC), and each of these components...  Read full blog post.
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Bioinformatics

Gene Symbol TNNI3