![Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Analysis of human lymph node lysate (35ug per lane, RIPA buffer) at 0.5ug/ml. Band detected at ~35kDa and ~28kDa. (Expected MW of 35.5kDa according to NP_003692.1 and of 27.7kDa according to NP_143026.1)](http://images.novusbio.com/fullsize/TRANCE-TNFSF11-RANK-L-Antibody-12A668-Azide-Free-Western-Blot-NB100-56593-img0008.jpg "Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Analysis of human lymph node lysate (35ug per lane, RIPA buffer) at 0.5ug/ml. Band detected at ~35kDa and ~28kDa. (Expected MW of 35.5kDa according to NP_003692.1 and of 27.7kDa according to NP_143026.1)"){kind=icon}
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ChIP, Flow, ICC/IF, IHC, ChIP, CyTOF-ready |
| Clone | 12A668 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE was used as immunogen (NP_003692). |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TNFSF11 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 35 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Sterile - filtered PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for TRANCE/TNFSF11/RANK L Antibody (NB100-56593)Find related products by research area.
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The diverse functions of RANKL/TRANCE/TNFSF11 RANKL (also known as TNF-related activation-induced cytokine), or receptor activator of nuclear factor-?B ligand, was first discovered as a key player in the RANKL/RANK/OPG osteoclast formation pathway. Osteoclasts are large multinucleate cells t... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - TRANCE was detected in immersion fixed mouse splenocytes using anti-human/mouse/rat mouse monoclonal antibody (Catalog # NB100-56512) for 1 hour at room temperature. Cells were stained using NL557 (red) fluorescent anti-mouse secondary antibodies (Catalog # NL007) and counterstained with DAPI (blue). Image using the standard format of this product.](http://images.novusbio.com/fullsize/TRANCE-TNFSF11-RANK-L-Antibody-12A668-Azide-Free-Immunocytochemistry-Immunofluorescence-NB100-56593-img0012.jpg "Immunocytochemistry/Immunofluorescence: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - TRANCE was detected in immersion fixed mouse splenocytes using anti-human/mouse/rat mouse monoclonal antibody (Catalog # NB100-56512) for 1 hour at room temperature. Cells were stained using NL557 (red) fluorescent anti-mouse secondary antibodies (Catalog # NL007) and counterstained with DAPI (blue). Image using the standard format of this product."){kind=icon}
![Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Formalin-fixed, paraffin-embedded human lymph node probed with Trance antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/TRANCE-TNFSF11-RANK-L-Antibody-12A668-Azide-Free-Immunohistochemistry-Paraffin-NB100-56593-img0011.jpg "Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Formalin-fixed, paraffin-embedded human lymph node probed with Trance antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min."){kind=icon}
![Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Analysis of transfected cell lysate was probed with 2 ug of Trance antibody.](http://images.novusbio.com/fullsize/TRANCE-TNFSF11-RANK-L-Antibody-12A668-Azide-Free-Western-Blot-NB100-56593-img0009.jpg "Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Analysis of transfected cell lysate was probed with 2 ug of Trance antibody."){kind=icon}
![Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Formalin-fixed, paraffin-embedded human liver stained with Trance antibody (1:500, 1 ug/ml), peroxidase-conjugate and DAB chromogen. A 2 hr incubation at RT was used.](http://images.novusbio.com/fullsize/TRANCE-TNFSF11-RANK-L-Antibody-12A668-Azide-Free-Immunohistochemistry-Paraffin-NB100-56593-img0010.jpg "Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Formalin-fixed, paraffin-embedded human liver stained with Trance antibody (1:500, 1 ug/ml), peroxidase-conjugate and DAB chromogen. A 2 hr incubation at RT was used."){kind=icon}
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")