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TRANCE/TNFSF11/RANK L Antibody (12A668) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, ChIP
Clone
12A668
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-56593AF350
Alexa Fluor 405
NB100-56593AF405
Alexa Fluor 488
NB100-56593AF488
Alexa Fluor 532
NB100-56593AF532
Alexa Fluor 594
NB100-56593AF594
Alexa Fluor 647
NB100-56593AF647
Alexa Fluor 700
NB100-56593AF700
Alexa Fluor 750
NB100-56593AF750
Allophycocyanin
NB100-56593APC
Biotin
NB100-56512B
DyLight 350
NB100-56593UV
DyLight 405
NB100-56593V
DyLight 488
NB100-56593G
DyLight 550
NB100-56593R
DyLight 594
NB100-56512DL594
DyLight 650
NB100-56593C
DyLight 680
NB100-56593FR
DyLight 755
NB100-56593IR
FITC
NB100-56593F
HRP
NB100-56593H
Janelia Fluor 549
NB100-56512JF549
PE
NB100-56593PE
Unconjugated
NB100-56512
mFluor Violet 450 SE
NB100-56593MFV450
mFluor Violet 500 SE
NB100-56593MFV500
mFluor Violet 610 SE
NB100-56593MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-56512
BSA Free
NB100-56512
BSA Free
NB100-56512SS
Azide and BSA Free
NB100-56593SS
Azide and BSA Free
NB100-56593
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TRANCE/TNFSF11/RANK L Antibody (12A668) [Janelia Fluor® 646] Summary

Immunogen
A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE/TNFSF11/RANK L was used as immunogen.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNFSF11
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA
  • Flow (Cell Surface)
  • Immunoassay
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for TRANCE/TNFSF11/RANK L Antibody (12A668) [Janelia Fluor® 646]

  • CD254 antigen
  • CD254
  • ODF
  • OPGL
  • OPGLOPTB2
  • Osteoclast differentiation factor
  • Osteoprotegerin ligand
  • RANK L
  • RANKL
  • RANKLreceptor activator of nuclear factor kappa B ligand
  • Receptor activator of nuclear factor kappa-B ligand
  • sOdf
  • TNF-related activation-induced cytokine
  • TNFSF11
  • TRANCE
  • TRANCEODFhRANKL2
  • tumor necrosis factor (ligand) superfamily, member 11
  • tumor necrosis factor ligand superfamily member 11

Background

TRANCE/OPGL/RANKL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells (1-2). RANK ligand (RANKL/TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). TRANCE/RANKL is also important in T and B-cell maturation (3). As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis (4). OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TRANCE/TNFSF11/RANK L ...
NB100-56512JF646
TRANCE/TNFSF11/RANK L Antibody (12A668) [Janelia Fluor® 646]
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, ChIP

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Video Protocols

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Secondary Antibodies

 

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The diverse functions of RANKL/TRANCE/TNFSF11
RANKL (also known as TNF-related activation-induced cytokine), or receptor activator of nuclear factor-?B ligand, was first discovered as a key player in the RANKL/RANK/OPG osteoclast formation pathway. Osteoclasts are large multinucleate cells t...  Read full blog post.
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Bioinformatics

Gene Symbol TNFSF11