Home » TOP2A » TOP2A Antibodies » TOP2A Antibody (680130) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

TOP2A Antibody (680130) [Unconjugated]

 Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human TOP2A Monoclonal Antibody (Catalog # MAB6540) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TOP2A at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 0.5 ...read more
TOP2A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human TOP2A Monoclonal Antibody (Catalog # MAB6540) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
680130
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6540-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

TOP2A Antibody (680130) [Unconjugated] Summary

Specificity
Detects human TOP2A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant TOP2B from human or mouse is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TOP2A
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TOP2A Antibody (680130) [Unconjugated]

  • DNA topoisomerase (ATP-hydrolyzing)
  • DNA topoisomerase 2-alpha
  • DNA topoisomerase II, 170 kD
  • DNA topoisomerase II, alpha isozyme
  • EC 5.99.1.3
  • TOP2
  • TOP2A
  • TOP2DNA gyrase
  • topoisomerase (DNA) II alpha (170kD)
  • topoisomerase (DNA) II alpha 170kDa
  • TP2A

Background

DNA topoisomerase 2-alpha (TOP2A; also DNA topoisomerase II, alpha isozyme) is a 170 kDa member of the type II topoisomerase family. Human TOP2A is 1531 amino acids (aa) in length, and splicing variants produce three additional isoforms which have substitutions following Lys321, Gln355, or Ala401. TOP2A contains a histidine kinase-like ATPases domain (aa 80-193), a TopoIIA Trans ScTopoIIA domain (aa 265-417), a TOPRIM TopoIIA: topoisomerase-primase domain (aa 455-575), a DNA Topoisomerase IV domain (aa 697-1169), and a NES (aa 1018-1028). There are multiple potential Ser/Thr phosphorylation sites. Human TOP2A shares 89% aa sequence identity with mouse TOP2A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CCL18/PARC [Biotin]
DY394
N/A CCL18/PARC [Biotin]
(34 Publications)
Species: Hu
Applications: ELISA
PARC Antibody -
BSA Free
NBP1-77222
Immunocytochemistry/ Immunofluorescence PARC Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence PARC Antibody - BSA Free
Western Blot PARC Antibody - BSA Free
Species: Hu, Mu
Applications: ELISA, ICC/IF, WB
ErbB2/Her2
[Unconjugated]
1129-ER
SDS-PAGE ErbB2/Her2 [Unconjugated]
Bioactivity ErbB2/Her2 [Unconjugated]
(58 Publications)
Species: Hu
Applications: BA
NOV/CCN3 Antibody
AF1640
Immunohistochemistry NOV/CCN3 Antibody
(4 Publications)
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, WB
Plexin A1 Antibody
[Unconjugated]
AF4309
Immunohistochemistry Plexin A1 Antibody [Unconjugated]
Immunocytochemistry Plexin A1 Antibody [Unconjugated]
Western Blot Plexin A1 Antibody [Unconjugated]
(11 Publications)
Species: Hu, Mu
Applications: ICC, IHC, WB
RPL10 Antibody
NBP1-84037
Immunohistochemistry-Paraffin RPL10 Antibody
Immunohistochemistry-Paraffin RPL10 Antibody
Immunohistochemistry-Paraffin RPL10 Antibody
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Tyrosinase
Antibody (JA52-11)
NBP2-67232
Immunocytochemistry/ Immunofluorescence Tyrosinase Antibody (JA52-11)
Immunocytochemistry/ Immunofluorescence Tyrosinase Antibody (JA52-11)
Immunohistochemistry-Paraffin Tyrosinase Antibody (JA52-11)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
RFC2 Antibody
(OTI2B2) - Azide and ...
NBP2-73857
Western Blot RFC2 Antibody (OTI2B2) - Azide and BSA Free
Flow Cytometry RFC2 Antibody (OTI2B2) - Azide and BSA Free
Immunohistochemistry RFC2 Antibody (OTI2B2) - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, IHC, WB
RFC1 Antibody
[Unconjugated]
AF6457
Western Blot RFC1 Antibody [Unconjugated]
Species: Hu
Applications: WB
RFC4 Antibody
(OTI1A8)
NBP2-45946
Immunocytochemistry/ Immunofluorescence RFC4 Antibody (OTI1A8)
Immunohistochemistry RFC4 Antibody (OTI1A8)
Immunohistochemistry RFC4 Antibody (OTI1A8)
Species: Ca, Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Rad51 Antibody
(14B4)
NB100-148
Western Blot Rad51 Antibody (14B4)
Western Blot Rad51 Antibody (14B4)
Western Blot Rad51 Antibody (14B4)
(5 Reviews)
(65 Publications)
Species: Ch, Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, KD, PLA, WB
EGFR Antibody
[Unconjugated]
AF231
Immunohistochemistry EGFR Antibody [Unconjugated]
Immunocytochemistry EGFR Antibody [Unconjugated]
Western Blot EGFR Antibody [Unconjugated]
(30 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
ATP6V0A1 Antibody
NBP1-89342
Immunocytochemistry/ Immunofluorescence ATP6V0A1 Antibody
Immunohistochemistry-Paraffin ATP6V0A1 Antibody
Immunohistochemistry-Paraffin ATP6V0A1 Antibody
(1 Review)
(13 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
Topoisomerase I
Antibody
NBP1-90365
Immunocytochemistry/ Immunofluorescence Topoisomerase I Antibody
Immunohistochemistry-Paraffin Topoisomerase I Antibody
Immunohistochemistry-Paraffin Topoisomerase I Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PLA, WB
TOP2B Antibody
NBP1-89527
Immunocytochemistry/ Immunofluorescence TOP2B Antibody
Western Blot TOP2B Antibody
Western Blot TOP2B Antibody
(2 Reviews)
(6 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, Simple Western, WB
NHE6/SLC9A6
Antibody
NBP2-38877
Immunocytochemistry/ Immunofluorescence NHE6/SLC9A6 Antibody
Immunohistochemistry-Paraffin NHE6/SLC9A6 Antibody
Immunohistochemistry-Paraffin NHE6/SLC9A6 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
HSP60 Antibody -
BSA Free
NBP1-77397
Immunocytochemistry/ Immunofluorescence HSP60 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence HSP60 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence HSP60 Antibody - BSA Free
(4 Reviews)
(6 Publications)
Species: Hu, Mu
Applications: Flow, IB, ICC/IF, IHC, IHC-P, KD, Simple Western, WB
TOP2A Antibody
(680130) [Unconjugate
MAB6540
TOP2A Antibody (680130) [Unconjugated]
Species: Hu
Applications: WB, ICC/IF

Publications for TOP2A Antibody (MAB6540) (0)

There are no publications for TOP2A Antibody (MAB6540).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TOP2A Antibody (MAB6540) (0)

There are no reviews for TOP2A Antibody (MAB6540). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TOP2A Antibody (MAB6540) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional TOP2A Products

Blogs on TOP2A

There are no specific blogs for TOP2A, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TOP2A Antibody (680130) [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol TOP2A
Uniprot