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TMEFF2/Tomoregulin-2 Antibody

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Western Blot: TMEFF2/Tomoregulin-2 Antibody [NBP1-84362] - Analysis in human cell line U-87 MG.
Immunohistochemistry-Paraffin: TMEFF2/Tomoregulin-2 Antibody [NBP1-84362] - Staining of human prostate shows weak granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: TMEFF2/Tomoregulin-2 Antibody [NBP1-84362] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: TMEFF2/Tomoregulin-2 Antibody [NBP1-84362] - Staining of human Cerebellum shows moderate granular cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: TMEFF2/Tomoregulin-2 Antibody [NBP1-84362] - Staining of human Cerebral cortex shows moderate granular cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: TMEFF2/Tomoregulin-2 Antibody [NBP1-84362] - Staining of human Skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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TMEFF2/Tomoregulin-2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FPTSLSDCQTPTGWNCSGYDDRENDLFLCDTNTCKFDGECLRIGDTVTCVCQFKCNNDYVPVCGSNGESYQNECYLRQAACKQQSEILVVSEGSCATDAGSGSGDGVHEGSGETSQKETSTCDICQFGAECDEDA
Predicted Species
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TMEFF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TMEFF2/Tomoregulin-2 Protein (NBP1-84362PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TMEFF2/Tomoregulin-2 Antibody

  • CT120.2
  • HPP1member 2
  • Hyperplastic polyposis protein 1
  • TENB2
  • TMEFF2
  • Tomoregulin2
  • Tomoregulin-2
  • TPEFtomoregulin
  • TR-2
  • transmembrane protein TENB2
  • transmembrane protein with EGF-like and two follistatin-like domains 2
  • Transmembrane protein with EGF-like and two follistatin-like domains
  • TRtomoregulin-2

Background

TMEFF2(transmembrane protein with EGF-like and two follistatin-like domains 2), a gene encoding a plasma membrane protein with two follistatin-like domains and one epidermal growth factor-like domain, had limited normal tissue distribution and was highly overexpressed in prostate cancer. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation. Down-regulated in tumor cell lines in response to a high level of methylation in the 5' region. Clone J4B6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TMEFF2 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TMEFF2