![Immunohistochemistry-Paraffin: TGF-alpha Antibody (SPM357) [NBP3-07354] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with TGF-alpha Mouse Monoclonal Antibody (SPM357)](http://images.novusbio.com/fullsize/TGF-alpha-Antibody-SPM357-Immunohistochemistry-Paraffin-NBP3-07354-img0001.jpg "Immunohistochemistry-Paraffin: TGF-alpha Antibody (SPM357) [NBP3-07354] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with TGF-alpha Mouse Monoclonal Antibody (SPM357)")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | Flow, ICC/IF, IHC |
| Clone | SPM357 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08270) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF-alpha (Uniprot: P01135) |
| Localization | Cytoplasmic and Extracellular (secreted) |
| Specificity | This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TGFA |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution for a specific application should be determined. |
| Theoretical MW | 6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for TGF-alpha Antibody (NBP3-07354)Find related products by research area.
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