![Immunohistochemistry-Paraffin: TGF-alpha Antibody (P/T1) - Azide and BSA Free [NBP2-34702] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with TGF alpha Monoclonal Antibody (P/T1).](http://images.novusbio.com/fullsize/TGF-alpha-Antibody-P-T1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34702-img0001.jpg "Immunohistochemistry-Paraffin: TGF-alpha Antibody (P/T1) - Azide and BSA Free [NBP2-34702] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with TGF alpha Monoclonal Antibody (P/T1).")
| Reactivity | Hu, Rb, ZeSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | P/T1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34299). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGF-alpha (Uniprot: P01135) |
| Localization | Cytoplasmic and Extracellular (secreted) |
| Specificity | This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this monoclonal antibody is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TGFA |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for TGF-alpha Antibody (NBP2-34702)Find related products by research area.
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| Gene Symbol | TGFA |
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