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TBCC Antibody (OTI4C4)

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Western Blot: TBCC Antibody (4C4) [NBP2-02037] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-TBCC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western Blot: TBCC Antibody (4C4) [NBP2-02037] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-TBCC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: ...read more
Immunocytochemistry/ Immunofluorescence: TBCC Antibody (4C4) [NBP2-02037] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TBCC.
Immunohistochemistry-Paraffin: TBCC Antibody (4C4) [NBP2-02037] - Staining of paraffin-embedded Human prostate tissue using anti-TBCC mouse monoclonal antibody.
Western Blot: TBCC Antibody (4C4) [NBP2-02037] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBCC (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: TBCC Antibody (4C4) [NBP2-02037] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TBCC mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TBCC Antibody (4C4) [NBP2-02037] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TBCC mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TBCC Antibody (4C4) [NBP2-02037] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TBCC mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TBCC Antibody (4C4) [NBP2-02037] - Staining of paraffin-embedded Human colon tissue using anti-TBCC mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TBCC Antibody (4C4) [NBP2-02037] - Staining of paraffin-embedded Human pancreas tissue using anti-TBCC mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI4C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74467AF350
Alexa Fluor 405
NBP2-74467AF405
Alexa Fluor 488
NBP2-74467AF488
Alexa Fluor 532
NBP2-74467AF532
Alexa Fluor 594
NBP2-74467AF594
Alexa Fluor 647
NBP2-74467AF647
Alexa Fluor 700
NBP2-74467AF700
Alexa Fluor 750
NBP2-74467AF750
Biotin
NBP2-74467B
CoraFluor 1
NBP2-74467CL1
DyLight 350
NBP2-74467UV
DyLight 405
NBP2-74467V
DyLight 488
NBP2-74467G
DyLight 550
NBP2-74467R
DyLight 594
NBP2-74467DL594
DyLight 650
NBP2-74467C
DyLight 680
NBP2-74467FR
DyLight 755
NBP2-74467IR
FITC
NBP2-74467F
HRP
NBP2-74467H
Janelia Fluor 525
NBP2-74467JF525
Janelia Fluor 549
NBP2-74467JF549
Janelia Fluor 646
NBP2-74467JF646
Janelia Fluor 669
NBP2-74467JF669
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-74467
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TBCC Antibody (OTI4C4) Summary

Immunogen
Full length human recombinant protein of human TBCC(NP_003183) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TBCC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TBCC Antibody (OTI4C4)

  • CFCtubulin-specific chaperone c
  • tubulin folding cofactor C
  • Tubulin-folding cofactor C

Background

Cofactor C is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TBCC
Entrez