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TAK1 Antibody (491840)

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Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human TAK1 Monoclonal Antibody (Catalog # MAB5307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TAK1 at approximately 80 to 82 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
491840
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5307-SP
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TAK1 Antibody (491840) Summary

Immunogen
E. coli-derived recombinant human TAK1 isoform 1A
Met1-Ser579
Accession # Q5U0D0
Specificity
Detects endogenous human TAK1 in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MAP3K7
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB5307 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TAK1 Antibody (491840)

  • EC 2.7.11
  • EC 2.7.11.25
  • MAP3K7
  • MEKK7
  • mitogen-activated protein kinase kinase kinase 7
  • TAK1
  • TAK1/MAP3K7
  • TAK1TGF1a
  • TGF1a
  • TGF-beta activated kinase 1
  • TGF-beta-activated kinase 1
  • Transforming growth factor-beta-activated kinase 1

Background

TGF-beta-activated kinase 1 (TAK1; also MAP3K7) is a member of the STE Ser/Thr protein kinase family and the MAP kinase kinase kinase subfamily. Human TAK1 is 606 amino acids (aa) in length and contains a protein kinase domain at aa 36‑291. There are three isoforms for TAK1. Isoform 1B is the largest isoform. Isoform 1A has a 27 aa deletion corresponding to aa 404‑430 in isoform 1B, and isoform 1C has an 88 aa deletion corresponding to aa 519‑606 in isoform 1B. TAK1 activity has been implicated during TGF beta -induced and Ras-dependent tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TAK1 Antibody
(491840)
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TAK1 Antibody (491840)
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Publications for TAK1 Antibody (MAB5307)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MAP3K7
Uniprot