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Symplekin Antibody (4C2)

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Western Blot: Symplekin Antibody (4C2) [H00008189-M03] - Analysis of SYMPK expression in transfected 293T cell line by SYMPK monoclonal antibody (M03), clone 4C2. Lane 1: SYMPK transfected lysatE (58.9 KDa). Lane 2: Non-transfected lysate.
Western Blot: Symplekin Antibody (4C2) [H00008189-M03] - Analysis of SYMPK expression in transfected 293T cell line by SYMPK monoclonal antibody (M03), clone 4C2. Lane 1: SYMPK transfected lysatE (58.9 KDa). Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: Symplekin Antibody (4C2) [H00008189-M03] - Analysis of monoclonal antibody to SYMPK on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: Symplekin Antibody (4C2) [H00008189-M03] - Analysis of monoclonal antibody to SYMPK on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml
Western Blot: Symplekin Antibody (4C2) [H00008189-M03] - SYMPK expression in COLO 320 HSR ( Cat # L020V1 ).
ELISA: Symplekin Antibody (4C2) [H00008189-M03] - Detection limit for recombinant GST tagged SYMPK is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
4C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Symplekin Antibody (4C2) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
SYMPK (AAH30214, 1 a.a. ~ 533 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASGSGDSVTRRSVASQFFTQEEGPGIDGMTTSERVVDLLNQAALITNDSKITVLKQVQELIINKDPTLLDNFLDEIIAFQADKSIEVRKFVIGFIEEACKRDIELLLKLIANLNMLLRDENVNVVKKAILTMTQLYKVALQWMVKSRVISELQEACWDMVSAMAGDIILLLDSDNDGIRTHAIKFVEGLIVTLSPRMADSEIPRRQEHDISLDRIPRDHPYIQYNVLWEEGKAALEQLLKFMVHPAISSINLTTALGSLANIARQRPMFMSEVIQAYETLHANLPPTLAKSQVSSVRKNLKLHLLSVLKHPASLEFQAQITTLLVDLGTPQAEIARNMPSSKDTRKRPRDDSDSTLKKMKLEPNLGEDDEDKDLEPGPSGTSKASAQISGQSDTDITAEFLQPLLTPDNVANLVLISMVYLPEAMPASFQAIYTPVESAGTEAQIKHLARLMATQMTAAGLGPGVEQTKQCKEEPKEEKVVKTESVLIKRRLSAQGQAISVVGSLSSMSPLEEEAPQAKRRPEPIIPVTQPR
Localization
Cytoplasm, cytoskeleton. Cell junction, adherens junction; Cytoplasmic side. Cell junction; Cytoplasmic side. Nucleus, nucleoplasm. Note=Cytoplasmic face of adhesion plaques (major) and nucleoplasm (minor) (in cells with TJ). Nucleoplasm (in cells without
Specificity
SYMPK - symplekin
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SYMPK
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Symplekin Antibody (4C2)

  • FLJ27092
  • SPKSYM
  • symplekin

Background

This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Bioinformatics

Gene Symbol SYMPK
Entrez
OMIM
Uniprot