Recombinant Human SP-D His Protein Summary
Description |
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 224-375 of Human SP-D Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSVASLRQQ VEALQGQVQH LQAAFSQYKK VELFPNGQSV GEKIFKTAGF VKPFTEAQLL CTQAGGQLAS PRSAAENAAL QQLVVAKNEA AFLSMTDSKT EGKFTYPTGE SLVYSNWAPG EPNDDGGSED CVEIFTNGKW NDRACGEKRL VVCEF |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
SFTPD |
Purity |
>85%, by SDS-PAGE |
Applications/Dilutions
Dilutions |
|
Application Notes |
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays. |
Theoretical MW |
18.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
>85%, by SDS-PAGE |
Alternate Names for Recombinant Human SP-D His Protein
Background
Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular head regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 88 ng/ml in healthy individuals (2).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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