![Western Blot: SMNDC1 Antibody [NBP1-47302] - Whole cell lysates from ovarian surface epithial cells (OSE), ovarian cancer cell line A2780, breast cancer cell line MCF7. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SMNDC1-Antibody-Western-Blot-NBP1-47302-img0003.jpg "Western Blot: SMNDC1 Antibody [NBP1-47302] - Whole cell lysates from ovarian surface epithial cells (OSE), ovarian cancer cell line A2780, breast cancer cell line MCF7. Image submitted by a verified customer review.")
| Reactivity | Hu, Mu, Rt, Bv, Pm-Or, XpSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen for this product maps to a region between residue 188 and 238 of human Splicing Factor 30 using the numbering given in entry NP_005862.1 (GeneID 10285). |
| Predicted Species | Rat (100%), Xenopus (100%), Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SMNDC1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | SMNDC1 antibody validated for WB from a verified customer review. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Prabir Chakraborty |
WB | Human | 06/02/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for SMNDC1 Antibody (NBP1-47302)Find related products by research area.
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![Western Blot: SMNDC1 Antibody [NBP1-47302] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SMNDC1 antibody NBP1-47302 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SPF30 was also immunoprecipitated by rabbit anti-SMNDC1 antibody NBP1-47301, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/SMNDC1-Antibody-Western-Blot-NBP1-47302-img0001.jpg "Western Blot: SMNDC1 Antibody [NBP1-47302] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SMNDC1 antibody NBP1-47302 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SPF30 was also immunoprecipitated by rabbit anti-SMNDC1 antibody NBP1-47301, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
