![Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873] - Analysis in human skeletal muscle and liver tissues using NBP2-52873 antibody. Corresponding SIX1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SIX1-Antibody-CL0185-Immunohistochemistry-Paraffin-NBP2-52873-img0010.jpg "Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873] - Analysis in human skeletal muscle and liver tissues using NBP2-52873 antibody. Corresponding SIX1 RNA-seq data are presented for the same tissues.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KD |
| Clone | CL0185 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: CFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDS |
| Predicted Species | Mouse (99%), Rat (99%). Backed by our 100% Guarantee. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SIX1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Protein A purified |
![Immunohistochemistry PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Immunohistochemistry_16650.jpg)
![Western Blot PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Western_Blot_16656.jpg)
| Publication using NBP2-52873 | Applications | Species |
|---|---|---|
| Jaime OG, Arias J, Pavani S et al. SIX1+PAX3+ identify a progenitor for myogenic lineage commitment from hPSCs Development (Cambridge, England) 2023-06-27 [PMID: 37366057] (IHC, ICC/IF, Human) | IHC, ICC/IF | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for SIX1 Antibody (NBP2-52873)Find related products by research area.
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| Gene Symbol | SIX1 |
![Western Blot: SIX1 Antibody (CL0185) [NBP2-52873] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATAD2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/SIX1-Antibody-CL0185-Western-Blot-NBP2-52873-img0005.jpg "Western Blot: SIX1 Antibody (CL0185) [NBP2-52873] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATAD2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![Immunocytochemistry/Immunofluorescence: SIX1 Antibody (CL0185) [NBP2-52873] - Staining in RH30 cell line with Anti-SIX1 monoclonal antibody, showing spotty nuclear staining in green. Microtubule probes are visualized in red (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SIX1-Antibody-CL0185-Immunocytochemistry-Immunofluorescence-NBP2-52873-img0001.jpg "Immunocytochemistry/Immunofluorescence: SIX1 Antibody (CL0185) [NBP2-52873] - Staining in RH30 cell line with Anti-SIX1 monoclonal antibody, showing spotty nuclear staining in green. Microtubule probes are visualized in red (where available). Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873] - Staining of human liver shows absence of immunoreactivity.](http://images.novusbio.com/fullsize/SIX1-Antibody-CL0185-Immunohistochemistry-Paraffin-NBP2-52873-img0002.jpg "Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873] - Staining of human liver shows absence of immunoreactivity.")
![Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873] - Staining of human striated muscle shows strong nuclear immunoreactivity in the myocytes.](http://images.novusbio.com/fullsize/SIX1-Antibody-CL0185-Immunohistochemistry-Paraffin-NBP2-52873-img0003.jpg "Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873] - Staining of human striated muscle shows strong nuclear immunoreactivity in the myocytes.")
![Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873] - Staining of human endocervix shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SIX1-Antibody-CL0185-Immunohistochemistry-Paraffin-NBP2-52873-img0006.jpg "Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873] - Staining of human endocervix shows strong nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873] - Staining of human salivary gland shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SIX1-Antibody-CL0185-Immunohistochemistry-Paraffin-NBP2-52873-img0007.jpg "Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873] - Staining of human salivary gland shows strong nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873] - Staining of human skeletal muscle shows strong nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/SIX1-Antibody-CL0185-Immunohistochemistry-Paraffin-NBP2-52873-img0008.jpg "Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873] - Staining of human skeletal muscle shows strong nuclear positivity in myocytes.")
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![Immunocytochemistry/ Immunofluorescence Pax3/Pax7 Antibody (274212) [Unconjugated]](https://images.novusbio.com/images/mab2457_human-mouse-pax3-pax7-mab-clone-274212-41202412512639.jpg)
![Immunocytochemistry Pax2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Pax2_AF3364_Immunocytochemistry_14405.jpg)
![Western Blot Pax2 Antibody [Unconjugated]](https://images.novusbio.com/images2/af3364_human-pax2-affinity-purified-polyclonal-ab-western-blot-145202183852.jpg)
![Immunohistochemistry Pax2 Antibody [Unconjugated]](https://images.novusbio.com/images2/af3364_human-pax2-affinity-purified-polyclonal-ab-immunohistochemistry-192021142455.jpg)
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")