Home » Siglec-1/CD169 » Siglec-1/CD169 Antibodies » Siglec-1/CD169 Antibody (ED3) [PE/Atto594]
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Siglec-1/CD169 Antibody (ED3) [PE/Atto594]

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, ICC/IF, IHC, IP
Clone
ED3
Clonality
Monoclonal
Host
Mouse
Conjugate
PE/Atto594

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-65301AF350
Alexa Fluor 405
NB100-65301AF405
Alexa Fluor 488
NB100-65301AF488
Alexa Fluor 532
NB100-65301AF532
Alexa Fluor 594
NB100-65301AF594
Alexa Fluor 647
NB100-65301AF647
Alexa Fluor 700
NB100-65301AF700
Alexa Fluor 750
NB100-65301AF750
Allophycocyanin
NB100-65301APC
Allophycocyanin/Cy7
NB100-65301APCCY7
CoraFluor 1
NB100-65301CL1
DyLight 350
NB100-65301UV
DyLight 405
NB100-65301V
DyLight 488
NB100-65301G
DyLight 550
NB100-65301R
DyLight 594
NB100-65301DL594
DyLight 650
NB100-65301C
DyLight 680
NB100-65301FR
DyLight 755
NB100-65301IR
Janelia Fluor 525
NB100-65301JF525
Janelia Fluor 549
NB100-65301JF549
Janelia Fluor 646
NB100-65301JF646
Janelia Fluor 669
NB100-65301JF669
PE
NB100-65301PE
PE/Cy5.5
NB100-65301PECY55
PE/Cy7
NB100-65301PECY7
PerCP
NB100-65301PCP
Unconjugated
NB100-65301
mFluor Violet 450 SE
NB100-65301MFV450
mFluor Violet 500 SE
NB100-65301MFV500
mFluor Violet 610 SE
NB100-65301MFV610
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Siglec-1/CD169 Antibody (ED3) [PE/Atto594] Summary

Immunogen
Rat Spleen cell homogenate
Specificity
NB100-65301 recognizes the rat CD169 cell surface antigen, a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SIGLEC1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for Siglec-1/CD169 Antibody (ED3) [PE/Atto594]

  • CD169
  • FLJ00051
  • sialic acid binding Ig-like lectin 1, sialoadhesin
  • sialoadhesin
  • Siglec1
  • Siglec-1

Background

The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Siglec-1/CD169
Antibody (ED3) [PE/At ...
NB100-65301PEATT594
Siglec-1/CD169 Antibody (ED3) [PE/Atto594]
Species: Rt
Applications: Flow, ICC/IF, IHC, IP

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SIGLEC1