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Serpin E1/PAI-1 Antibody [Biotin]

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Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Serpin E1/PAI‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3828) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. A specific band was detected for Serpin E1/PAI‑1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions.
Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Serpin E1/PAI‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3828) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF3828
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF3828
Carrier Free
AF3828
Carrier Free
AF3828-SP
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Serpin E1/PAI-1 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Serpin E1/PAI‑1
Leu24-Ser402
Accession # AAH54091
Specificity
Detects mouse Serpin E1/PAI‑1 in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Serpin E1 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) Serpin E2 and rmSerpin I1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SERPINE1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin E1/PAI-1 Antibody [Biotin]

  • Endothelial plasminogen activator inhibitor
  • Nexin
  • PAI1
  • PAI-1
  • PAI1PAI-1
  • PAISerpin E1
  • PLANH1
  • PLANH1plasminogen activator inhibitor 1
  • serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1
  • Serpin E1
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

Background

Serpin E1, also known as Plasminogen Activator Inhibitor-1 (PAI-1), is the principal inhibitor of urokinase-type and tissue-type plasminogen activators. It plays a major role in many processes such as angiogenesis, tumor invasion, and obesity. The amino acid sequence of mouse PAI-1 is 89%, 79% and 76% identical to that of rat, human/chimpanzee and dog.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SERPINE1
Uniprot