![Western Blot: Serpin B5/Maspin Antibody (SERPINB5/4977) [NBP3-13734] - Western blot analysis of PC3 cell lysate using Serpin B5/Maspin antibody (SERPINB5/4977).](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-SERPINB5-4977-Western-Blot-NBP3-13734-img0001.jpg "Western Blot: Serpin B5/Maspin Antibody (SERPINB5/4977) [NBP3-13734] - Western blot analysis of PC3 cell lysate using Serpin B5/Maspin antibody (SERPINB5/4977).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, MA |
| Clone | SERPINB5/4977 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14137) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant fragment of human Serpin B5/Maspin protein (around aa1-200) (exact sequence is proprietary) (Uniprot: P36952) |
| Localization | Secreted, Extracellular space |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SERPINB5 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes |
| Theoretical MW | 42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody (SERPINB5/4977) [NBP3-13734] - Formalin-fixed, paraffin-embedded human skin stained with Serpin B5/Maspin antibody (SERPINB5/4977). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-SERPINB5-4977-Immunohistochemistry-Paraffin-NBP3-13734-img0002.jpg "Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody (SERPINB5/4977) [NBP3-13734] - Formalin-fixed, paraffin-embedded human skin stained with Serpin B5/Maspin antibody (SERPINB5/4977). Inset: PBS instead of primary antibody; secondary only negative control.")
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