![Western Blot: Septin-10 Antibody (2A12) [H00151011-M01] - SEPT10 monoclonal antibody (M01), clone 2A12 Analysis of SEPT10 expression in U-2 OS.](http://images.novusbio.com/fullsize/Septin-10-Antibody-2A12-Western-Blot-H00151011-M01-img0007.jpg "Western Blot: Septin-10 Antibody (2A12) [H00151011-M01] - SEPT10 monoclonal antibody (M01), clone 2A12 Analysis of SEPT10 expression in U-2 OS.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 2A12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | SEPT10 (AAH20502, 1 a.a. ~ 454 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASSEVARHLLFQSHMATKTTCMSSQGSDDEQIKRENIRSLTMSGHVGFESLPDQLVNRSIQQGFCFNILCVGETGIGKSTLIDTLFNTNFEDYESSHFCPNVKLKAQTYELQESNVQLKLTIVNTVGFGDQINKEESYQPIVDYIDAQFEAYLQEELKIKRSLFTYHDSRIHVCLYFISPTGHSLKTLDLLTMKNLDSKVNIIPVIAKADTVSKTELQKFKIKLMSELVSNGVQIYQFPTDDDTIAKVNAAMNGQLPFAVVGSMDEVKVGNKMVKARQYPWGVVQVENENHCDFVKLREMLICTNMEDLREQTHTRHYELYRRCKLEEMGFTDVGPENKPVSVQETYEAKRHEFHGERQRKEEEMKQMFVQRVKEKEAILKEAERELQAKFEHLKRLHQEERMKLEEKRRLLEEEIIAFSKKKATSEIFHSQSFLATGSNLRKDKDRKNSNFL |
| Specificity | 10-Sep - septin 10 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SEPT10 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
| Publication using H00151011-M01 | Applications | Species |
|---|---|---|
| Xu M, Takanashi M, Oikawa K et al. Identification of a novel role of Septin 10 in paclitaxel-resistance in cancers through a functional genomics screen. Cancer Sci. 2012-03-04 [PMID: 22320903] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Septin-10 Antibody (H00151011-M01)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Septin-10 Antibody (2A12) [H00151011-M01] - Analysis of monoclonal antibody to SEPT10 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Septin-10-Antibody-2A12-Immunocytochemistry-Immunofluorescence-H00151011-M01-img0005.jpg "Immunocytochemistry/Immunofluorescence: Septin-10 Antibody (2A12) [H00151011-M01] - Analysis of monoclonal antibody to SEPT10 on HeLa cell. Antibody concentration 10 ug/ml.")
![Western Blot: Septin-10 Antibody (2A12) [H00151011-M01] - Analysis of SEPT10 expression in transfected 293T cell line by SEPT10 monoclonal antibody (M01), clone 2A12.Lane 1: SEPT10 transfected lysate(52.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Septin-10-Antibody-2A12-Western-Blot-H00151011-M01-img0008.jpg "Western Blot: Septin-10 Antibody (2A12) [H00151011-M01] - Analysis of SEPT10 expression in transfected 293T cell line by SEPT10 monoclonal antibody (M01), clone 2A12.Lane 1: SEPT10 transfected lysate(52.6 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: Septin-10 Antibody (2A12) [H00151011-M01] - Detection limit for recombinant GST tagged SEPT10 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Septin-10-Antibody-2A12-Sandwich-ELISA-H00151011-M01-img0006.jpg "Sandwich ELISA: Septin-10 Antibody (2A12) [H00151011-M01] - Detection limit for recombinant GST tagged SEPT10 is approximately 1ng/ml as a capture antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")