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RRM1 Antibody (OTI5G5) - Azide and BSA Free

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Western Blot: RRM1 Antibody (OTI5G5) - Azide and BSA Free [NBP2-73947] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RRM1.
Immunohistochemistry: RRM1 Antibody (OTI5G5) - Azide and BSA Free [NBP2-73947] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: RRM1 Antibody (OTI5G5) - Azide and BSA Free [NBP2-73947] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: RRM1 Antibody (OTI5G5) - Azide and BSA Free [NBP2-73947] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI5G5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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RRM1 Antibody (OTI5G5) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 541-792 of human RRM1 (NP_001024) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RRM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
89.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for RRM1 Antibody (OTI5G5) - Azide and BSA Free

  • EC 1.17.4.1
  • R1
  • ribonucleoside-diphosphate reductase large subunit
  • Ribonucleoside-diphosphate reductase subunit M1
  • Ribonucleotide reductase large subunit
  • ribonucleotide reductase M1 polypeptide
  • ribonucleotide reductase M1
  • ribonucleotide reductase, large subunit
  • ribonucleotide reductase, R1 subunit
  • RIR1
  • RR1
  • RRM1

Background

RRM1 - ribonucleotide reductase M1 polypeptide

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RRM1