![Western Blot: RPP25 Antibody [NBP1-92349] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line SK-MEL-30](http://images.novusbio.com/fullsize/RPP25-Antibody-Western-Blot-NBP1-92349-img0005.jpg "Western Blot: RPP25 Antibody [NBP1-92349] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line SK-MEL-30")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: ASLSVLKNVPGLAILLSKDALDPRQPGYQPPNPHPGPSSPPAAPASKRSLGEPAAGEGSAKRSQPEP |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RPP25 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RPP25 |
![Immunocytochemistry/Immunofluorescence: RPP25 Antibody [NBP1-92349] - Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & microtubule organizing center.](http://images.novusbio.com/fullsize/RPP25-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92349-img0006.jpg "Immunocytochemistry/Immunofluorescence: RPP25 Antibody [NBP1-92349] - Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & microtubule organizing center.")
![Immunohistochemistry-Paraffin: RPP25 Antibody [NBP1-92349] - Staining of human testis shows moderate nuclear positivity in Leydig cells.](http://images.novusbio.com/fullsize/RPP25-Antibody-Immunohistochemistry-Paraffin-NBP1-92349-img0009.jpg "Immunohistochemistry-Paraffin: RPP25 Antibody [NBP1-92349] - Staining of human testis shows moderate nuclear positivity in Leydig cells.")
![Immunohistochemistry-Paraffin: RPP25 Antibody [NBP1-92349] - Staining of human colon shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RPP25-Antibody-Immunohistochemistry-Paraffin-NBP1-92349-img0002.jpg "Immunohistochemistry-Paraffin: RPP25 Antibody [NBP1-92349] - Staining of human colon shows moderate nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: RPP25 Antibody [NBP1-92349] - Staining of human colon shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RPP25-Antibody-Immunohistochemistry-Paraffin-NBP1-92349-img0004.jpg "Immunohistochemistry-Paraffin: RPP25 Antibody [NBP1-92349] - Staining of human colon shows moderate nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: RPP25 Antibody [NBP1-92349] - Staining of human kidney shows weak to moderate nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/RPP25-Antibody-Immunohistochemistry-Paraffin-NBP1-92349-img0007.jpg "Immunohistochemistry-Paraffin: RPP25 Antibody [NBP1-92349] - Staining of human kidney shows weak to moderate nuclear positivity in cells in tubules.")
![Immunohistochemistry-Paraffin: RPP25 Antibody [NBP1-92349] - Staining of human skeletal muscle shows no nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/RPP25-Antibody-Immunohistochemistry-Paraffin-NBP1-92349-img0008.jpg "Immunohistochemistry-Paraffin: RPP25 Antibody [NBP1-92349] - Staining of human skeletal muscle shows no nuclear positivity in myocytes.")
![Western Blot GBL Antibody [Unconjugated]](https://images.novusbio.com/images2/GBL_AF4004_Western_Blot_5381.jpg)
![Immunohistochemistry GBL Antibody [Unconjugated]](https://images.novusbio.com/images2/GBL_AF4004_Immunohistochemistry_19617.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")