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RPL14 Antibody

Images

 
Western Blot: RPL14 Antibody [NBP1-85385] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: RPL14 Antibody [NBP1-85385] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & endoplasmic reticulum.
Immunohistochemistry-Paraffin: RPL14 Antibody [NBP1-85385] - Staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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RPL14 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KKMRNRIIKNEVKKLQKAALLKASPKKAPGTKGTAAAAAAAAAAAAAAAKVPAKKITAASKKAPAQKVPAQKATGQKAAPAPKAQKGQKAPAQKAPAPKAS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPL14
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RPL14 Protein (NBP1-85385PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RPL14 Antibody

  • CAG-ISL 7
  • CAG-ISL-7
  • CTG-B33
  • hRL14
  • L14
  • MGC88594
  • MMRPL32,60S ribosomal protein L14
  • ribosomal protein L14
  • RL14

Background

RPL14, also known as Ribosomal Protein L14, is 215 amino acids long and approximately 23kDa. RPL14 is a cytoplasmic protein that is component of the large 60S subunit of the ribosome. RPL14 contains a bZIP-like domain. Current research surrounding RPL14 has shown possible connections with diseases and disorders such as oral cancer, anemia, Treacher Collins syndrome, squamous cell carcinoma, malaria, pancreatitis, and Congenital myasthenic syndrome. RPL14 interacts with other ribosomal proteins such as RPL11, RPL13, RPL17, RPL26 and RPL 27A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RPL14