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ROR alpha/NR1F1 Antibody (784652) [Unconjugated]

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Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Th17 cells were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (FAB3791P) and either (A) Mouse Anti-Human RORa/NR1F1 Monoclonal Antibody (Catalog # MAB8924) or (B) Mouse IgG1Isotype Control (MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012).
Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Th17 cells were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (FAB3791P) and either (A) Mouse Anti-Human RORa/NR1F1 Monoclonal ...read more
Jurkat cells were stained with Mouse Anti-Human ROR alpha /NR1F1 Monoclonal Antibody (Catalog # MAB8924, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications CyTOF-ready, Flow
Clone
784652
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC8924U-100UG
Alexa Fluor 405
IC8924V-100UG
Alexa Fluor 488
IC8924G-100UG
Alexa Fluor 532
FAB8924X
Alexa Fluor 594
IC8924T-100UG
Alexa Fluor 647
IC8924R-100UG
Alexa Fluor 700
IC8924N-100UG
Alexa Fluor 750
IC8924S-100UG
Allophycocyanin
FAB8924A
Biotin
FAB8924B
CoraFluor 1
FAB8924CL1
DyLight 350
FAB8924D
DyLight 405
FAB8924E
DyLight 488
FAB8924K
DyLight 550
FAB8924L
DyLight 594
FAB8924M
DyLight 650
FAB8924W
DyLight 680
FAB8924Y
DyLight 755
FAB8924Z
FITC
FAB8924F
HRP
FAB8924H
Janelia Fluor 525
FAB8924JF525
Janelia Fluor 549
FAB8924I
Janelia Fluor 646
FAB8924J
Janelia Fluor 669
FAB8924JF669
PerCP
FAB8924C
Phycoerythrin
IC8924P-100
Phycoerythrin
IC8924P-025
mFluor Violet 450 SE
FAB8924MFV450
mFluor Violet 500 SE
FAB8924MFV500
mFluor Violet 610 SE
FAB8924MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB8924-SP
Carrier Free
IC8924P-025
Carrier Free
IC8924V-100UG
Carrier Free
IC8924R-100UG
Carrier Free
IC8924G-100UG
Carrier Free
IC8924N-100UG
Carrier Free
IC8924U-100UG
Carrier Free
IC8924T-100UG
Carrier Free
IC8924S-100UG
Carrier Free
IC8924P-100
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ROR alpha/NR1F1 Antibody (784652) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human ROR alpha /NR1F1
Arg47-His163
Accession # P35398
Specificity
Detects human ROR alpha /NR1F1 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
RORA
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publication using
MAB8924 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROR alpha/NR1F1 Antibody (784652) [Unconjugated]

  • DKFZp686M2414
  • MGC119326
  • NR1F1
  • NR1F1MGC119329
  • nuclear receptor ROR-alpha
  • Nuclear receptor RZR-alpha
  • Nuclear receptor subfamily 1 group F member 1
  • RAR-related orphan receptor A
  • RAR-related orphan receptor alpha
  • retinoic acid receptor-related orphan receptor alpha
  • retinoid-related orphan receptor alpha
  • Retinoid-related orphan receptor-alpha
  • ROR alpha
  • ROR1
  • ROR2
  • ROR3
  • RORA
  • RZRA
  • RZR-ALPHA
  • RZRAROR-alpha
  • transcription factor RZR-alpha

Background

RORa (Retinoid-related Orphan Receptor alpha ) is a member of the NR1 nuclear hormone receptor family. ROR alpha is a DNA binding transcription factor, and can bind as a monomer or homodimer to hormone response elements to enhance expression.  Knockout mice implicate ROR alpha as being essential in the development of the cerebellum. It has also been reported to regulate lymphocyte development and play a role in Th17 differentiation. Human ROR alpha shares 99% amino sequence identity with mouse ROR alpha .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ROR alpha/NR1F1 Antibody (MAB8924)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RORA
Uniprot