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Recombinant Rat IGF-I/IGF-1 Protein, CF

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Summary
Reactivity RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Rat IGF-I/IGF-1 Protein, CF Summary

Details of Functionality
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.6-3 ng/mL.
Source
E. coli-derived rat IGF-I/IGF-1 protein
MHHHHHHN Rat IGF-I/IGF-1
(Gly23-Ala92)
Accession # AAA41386
N-terminus C-terminus
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Igf1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
8.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
4326-RG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat IGF-I/IGF-1 Protein, CF

  • IBP1
  • IGF1
  • IGF-1
  • IGF1A
  • IGFI
  • IGF-I
  • IGF-IA
  • IGF-IB
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • insulin-like growth factor IA
  • insulin-like growth factor IB
  • Mechano growth factor
  • MGF
  • Somatomedin A
  • Somatomedin C
  • somatomedin-C

Background

Insulin-like growth factor I (IGF-I or IGF-1), also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Rat IGF-I/IGF-1 is synthesized as four precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I/IGF-1 is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature rat IGF-I/IGF-1 shares 96% and 99% aa sequence identity with human and mouse IGF-I/IGF-1, respectively (2, 3), and exhibits cross-species activity. It shares 61% aa sequence identity with mature rat IGF-II/IGF-2. Circulating IGF-I/IGF-1 is produced by hepatocytes, while local IGF-I/IGF-1 is produced by many other tissues in which it has paracrine effects (1). IGF-I/IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (4). IGF-I/IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (5 - 8). It plays an important role in muscle regeneration and tumor progression (1, 9). IGF-I/IGF-1 binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (10). Heterodimers of insulin receptor/IGF-I R also exist and respond to IGF-I/IGF-1 but not to insulin stimulation (11). IGF-I/IGF-1 association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (12).

  1. Philippou, A. et al. (2007) In Vivo 21:45.
  2. Roberts, Jr., C.T. et al. (1987) Mol. Endocrinol. 1:243.
  3. Shimatsu, A. and P. Rotwein (1987) J. Biol. Chem. 262:7894.
  4. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
  5. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
  6. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
  7. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
  8. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
  9. Samani, A.A. et al. (2007) Endocr. Rev. 28:20.
  10. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
  11. Benyoucef, S. et al. (2007) Biochem. J. 403:603.
  12. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

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Recombinant Rat
IGF-I/IGF-1 Protein,
4326-RG
Recombinant Rat IGF-I/IGF-1 Protein, CF
Species: Rt
Applications: Bioactivity

Publications for IGF-I/IGF-1 (4326-RG)(10)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: Bioassay, In Vivo.


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Bioassay
(8)
In Vivo
(3)
All Applications
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Mouse
(2)
Rat
(9)
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Showing Publications 1 - 10 of 10.
Publications using 4326-RG Applications Species
B Karayay, H Olze, AJ Szczepek Degranulation of Murine Resident Cochlear Mast Cells: A Possible Factor Contributing to Cisplatin-Induced Ototoxicity and Neurotoxicity International Journal of Molecular Sciences, 2023-02-27;24(5):. 2023-02-27 [PMID: 36902051] (Bioassay, Mouse) Bioassay Mouse
AJ Szczepek, T Dudnik, B Karayay, V Sergeeva, H Olze, A Smorodchen Mast Cells in the Auditory Periphery of Rodents Brain Sci, 2020-10-01;10(10):. 2020-10-01 [PMID: 33019672] (Bioassay, Mouse, Rat) Bioassay Mouse, Rat
X Ding, W Yang, Q Ren, J Hu, S Yang, W Han, J Wang, X Wang, H Wang Serum IgG-induced microglial activation enhances neuronal cytolysis via the NO/sGC/PKG pathway in children with opsoclonus-myoclonus syndrome and neuroblastoma J Neuroinflammation, 2020-06-16;17(1):190. 2020-06-16 [PMID: 32546235] (Bioassay, Rat) Bioassay Rat
H Chen, H Zhu, J Yang, Y Zhu, J Mei, H Shen, K Liang, X Zhang Role of Programmed Cell Death 4 (PDCD4)-Mediated Akt Signaling Pathway in Vascular Endothelial Cell Injury Caused by Lower-Extremity Ischemia-Reperfusion in Rats Med. Sci. Monit., 2019-06-29;25(0):4811-4818. 2019-06-29 [PMID: 31253757] (In Vivo, Rat) In Vivo Rat
JJ Yuan, Q Zhang, CX Gong, FX Wang, JC Huang, GQ Yang, L Liu, K Zhou, R Xu, Q Chen, Y Zhou, XY Xiong, QW Yang Young plasma ameliorates aging-related acute brain injury after intracerebral hemorrhage Biosci. Rep., 2019-05-17;0(0):. 2019-05-17 [PMID: 31040201] (In Vivo, Rat) In Vivo Rat
H Zhu, C Xue, M Yao, H Wang, P Zhang, T Qian, S Zhou, S Li, B Yu, Y Wang, X Gu miR-129 controls axonal regeneration via regulating insulin-like growth factor-1 in peripheral nerve injury Cell Death Dis, 2018-06-18;9(7):720. 2018-06-18 [PMID: 29915198] (Bioassay, In Vivo, Rat) Bioassay, In Vivo Rat
Fornaro , Mara, Hinken , Aaron C, Needle , Saul, Hu , Erding, Trendelenburg , Anne-Ulr, Mayer , Angelika, Rosenstiel , Antonia, Chang , Calvin, Meier , Viktor, Billin , Andrew N, Becherer , J David, Brace , Arthur D, Evans , William, Glass , David J, Russell , Alan J Mechano-growth factor peptide, the COOH terminus of unprocessed insulin-like growth factor 1, has no apparent effect on myoblasts or primary muscle stem cells. Am J Physiol Endocrinol Metab, 2013-11-19;306(2):E150-6. 2013-11-19 [PMID: 24253050] (Bioassay, Rat) Bioassay Rat
Insulin-like growth factor-1 lowers spreading depression susceptibility and reduces oxidative stress. J. Neurochem., 2012-05-17;122(1):221-9. 2012-05-17 [PMID: 22524542] (Bioassay, Rat) Bioassay Rat
Asumda FZ, Chase PB Age-related changes in rat bone-marrow mesenchymal stem cell plasticity. BMC Cell Biol., 2011-10-12;12(0):44. 2011-10-12 [PMID: 21992089] (Bioassay, Rat) Bioassay Rat
Gilbert RE, Huang Q, Thai K, Advani SL, Lee K, Yuen DA, Connelly KA, Advani A Histone deacetylase inhibition attenuates diabetes-associated kidney growth: potential role for epigenetic modification of the epidermal growth factor receptor. Kidney Int., 2011-03-09;79(12):1312-21. 2011-03-09 [PMID: 21389970] (Bioassay, Rat) Bioassay Rat

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Bioinformatics

Gene Symbol Igf1
Uniprot