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Recombinant Mouse IGF-I/IGF-1 Protein, CF

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Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG) stimulates cell proliferation in a serum-free assay using the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.4-2 ng/mL.
1 µg/lane of Recombinant Mouse IGF-I/IGF-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 8 kDa.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse IGF-I/IGF-1 Protein, CF Summary

Details of Functionality
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.4-2 ng/mL.
Source
E. coli-derived mouse IGF-I/IGF-1 protein
Gly33-Ala102
Accession #
N-terminal Sequence
Gly33
Protein/Peptide Type
Recombinant Proteins
Gene
Igf1
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
7.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
791-MG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IGF-I/IGF-1 Protein, CF

  • IBP1
  • IGF1
  • IGF-1
  • IGF1A
  • IGFI
  • IGF-I
  • IGF-IA
  • IGF-IB
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • insulin-like growth factor IA
  • insulin-like growth factor IB
  • Mechano growth factor
  • MGF
  • Somatomedin A
  • Somatomedin C
  • somatomedin-C

Background

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I/IGF-1 is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I/IGF-1 is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I/IGF-1 shares 94% and 99% aa sequence identity with human and rat IGF-I/IGF-1, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF-II/IGF-2. Circulating IGF-I/IGF-1 is produced by hepatocytes, while local IGF-I/IGF-1 is produced by many other tissues in which it has paracrine effects (1). IGF-I/IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I/IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I/IGF-1 binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I/IGF-1 association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).

  1. Philippou, A. et al. (2007) In Vivo 21:45.
  2. Bell, G.I. et al. (1986) Nucleic Acids Res. 14:7873.
  3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
  4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
  5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
  6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
  7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
  8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
  9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
  10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

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791-MG
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Applications: Bioactivity

Publications for IGF-I/IGF-1 (791-MG)(74)

We have publications tested in 3 confirmed species: Mouse, Rat, Transgenic Mouse.

We have publications tested in 5 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo, Western Blot Control.


Filter By Application
Bioassay
(54)
Cell Culture
(3)
ELISA (Standard)
(3)
In Vivo
(12)
Western Blot Control
(1)
All Applications
Filter By Species
Mouse
(69)
Rat
(1)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 74. Show All 74 Publications.
Publications using 791-MG Applications Species
Andries, L;Kancheva, D;Masin, L;Scheyltjens, I;Van Hove, H;De Vlaminck, K;Bergmans, S;Claes, M;De Groef, L;Moons, L;Movahedi, K; Immune stimulation recruits a subset of pro-regenerative macrophages to the retina that promotes axonal regrowth of injured neurons Acta neuropathologica communications 2023-05-24 [PMID: 37226256] (In Vivo, Mouse) In Vivo Mouse
Haensel, D;Daniel, B;Gaddam, S;Pan, C;Fabo, T;Bjelajac, J;Jussila, AR;Gonzalez, F;Li, NY;Chen, Y;Hou, J;Patel, T;Aasi, S;Satpathy, AT;Oro, AE; Skin basal cell carcinomas assemble a pro-tumorigenic spatially organized and self-propagating Trem2+ myeloid niche Nature communications 2023-05-10 [PMID: 37164949] (Cell Culture, Mouse) Cell Culture Mouse
KW Southerlan, Y Xu, DT Peters, X Wei, X Lin, Y Xiang, K Fei, LA Olivere, JM Morowitz, J Otto, Q Dai, CD Kontos, Y Diao Pro-inflammatory macrophages impair skeletal muscle regeneration in ischemic-damaged limbs by inducing precocious differentiation of satellite cells bioRxiv : the preprint server for biology, 2023-04-03;0(0):. 2023-04-03 [PMID: 37066299] (Bioassay, Mouse) Bioassay Mouse
X Hong, Y Jian, S Ding, J Zhou, X Zheng, H Zhang, B Zhou, C Zhuang, J Wan, X Tong Kir4.1 channel activation in NG2 glia contributes to remyelination in ischemic stroke EBioMedicine, 2022-12-15;87(0):104406. 2022-12-15 [PMID: 36527899] (Bioassay, Mouse) Bioassay Mouse
R Lesage, MN Ferrao Bla, R Narcisi, T Welting, GJVM van Osch, L Geris An integrated in silico-in vitro approach for identifying therapeutic targets against osteoarthritis Bmc Biology, 2022-11-09;20(1):253. 2022-11-09 [PMID: 36352408] (Bioassay, Mouse) Bioassay Mouse
A Hiroto, WK Kim, A Pineda, Y He, DH Lee, V Le, AW Olson, J Aldahl, CH Nenninger, AJ Buckley, GQ Xiao, J Geradts, Z Sun Stromal androgen signaling acts as tumor niches to drive prostatic basal epithelial progenitor-initiated oncogenesis Nature Communications, 2022-11-02;13(1):6552. 2022-11-02 [PMID: 36323713] (Bioassay, Mouse) Bioassay Mouse
L Wang, Z Chen, Y Feng, R Wang, X Bai, W Liu, D Wang RNA-seq transcriptomic analysis of 4-octyl itaconate repressing myogenic differentiation Archives of biochemistry and biophysics, 2022-09-29;0(0):109420. 2022-09-29 [PMID: 36183843] (Bioassay, Mouse) Bioassay Mouse
MRF Gosselin, V Mournetas, M Borczyk, S Verma, A Occhipinti, J Róg, L Bozycki, M Korostynsk, SC Robson, C Angione, C Pinset, DC Gorecki Loss of full-length dystrophin expression results in major cell-autonomous abnormalities in proliferating myoblasts Elife, 2022-09-27;11(0):. 2022-09-27 [PMID: 36164827] (Bioassay, Mouse) Bioassay Mouse
ALM Caldwell, L Sancho, J Deng, A Bosworth, A Miglietta, JK Diedrich, MN Shokhirev, NJ Allen Aberrant astrocyte protein secretion contributes to altered neuronal development in multiple models of neurodevelopmental disorders Nature Neuroscience, 2022-08-30;25(9):1163-1178. 2022-08-30 [PMID: 36042312] (Bioassay, Mouse) Bioassay Mouse
SJ Cha, X Yu, BD Gregory, YS Lee, T Ishino, RO Opoka, CC John, M Jacobs-Lor Identification of Key Determinants of Cerebral Malaria Development and Inhibition Pathways MBio, 2022-01-25;0(0):e0370821. 2022-01-25 [PMID: 35073748] (In Vivo, Mouse) In Vivo Mouse
Show All 74 Publications.

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Bioinformatics

Gene Symbol Igf1
Uniprot