Reactivity | MuSpecies Glossary |
Applications | Bioactivity |
Format | Carrier-Free |
Details of Functionality | Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD155/PVR is coated at 5 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD96 (Catalog # 5690‑CD) that produces 50% optimal binding response is found to be approximately 0.015‑0.075 μg/mL. |
Source | Mouse myeloma cell line, NS0-derived mouse CD155/PVR protein Met1-Leu348, with a C-terminal 6-His tag |
Accession # | |
N-terminal Sequence | Asp29 |
Protein/Peptide Type | Recombinant Proteins |
Gene | Pvr |
Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note | <0.01 EU per 1 μg of the protein by the LAL method. |
Dilutions |
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Theoretical MW | 35.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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SDS-PAGE | 63‑80 kDa, reducing conditions |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions | Reconstitute at 100 μg/mL in PBS. |
Publication using 6909-CD | Applications | Species |
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Bellucci R, Nguyen HN, Martin A, Heinrichs S, Schinzel AC, Hahn WC, Ritz J Tyrosine kinase pathways modulate tumor susceptibility to natural killer cells. J. Clin. Invest., 2012-06-11;122(7):2369-83. 2012-06-11 [PMID: 22684105] (Flow Cytometry, Human) | Flow Cytometry | Human |
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