Recombinant Human Thrombospondin-1 Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Thrombospondin-1 (TSP-1) is a 150-180 kDa calcium-sensitive protein that is secreted as a disulfide-linked homotrimer. Within the Thrombospondin family, TSP-2 is also homotrimeric, while TSP-3, -4, and -5/COMP are homopentameric. TSP-1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 contains an N-terminal Laminin G-like globular domain, an extended central region with one vWFC domain, 3 TSP type 1 domains, 2 EGF-like domains, and 8 TSP type-3 domains, and a globular TSP C-terminal domain. Distinct regions of TSP-1 have been associated with binding to particular ECM or cellular molecules (1, 2). Mature human TSP-1 shares 95% amino acid sequence identity with mouse and rat TSP-1. TSP-1 counteracts the angiogenic, hypotensive, and anti-thrombotic effects of nitric oxide (NO) (3). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells (EC), and destabilizes adhesive contacts between EC (4-6). TSP-1’s actions on vascular smooth muscle cells support arterial tone and blood pressure in the presence of vasodilatory stimulation (7). It is released from activated platelets and enhances platelet aggregation and adhesion (8). TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF-beta and inducing release of the active cytokine from the latency associated peptide (LAP) (9, 10). TSP-1 dampens adaptive immune responses by inducing the differentiation of regulatory T cells and inhibiting TCR signaling (11, 12). In the nervous system, it promotes excitatory synapse formation (13, 14) and supports the integration of neural progenitor cells into the olfactory bulb (15). In addition, TSP-1 is released by apoptotic cells and promotes macrophage-mediated debris clearance (16).

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Bioassay, ELISA (Standard), ELISA Standard, Enzyme Assay, In Vivo.

Showing Publications 1 - 10 of 31. Show All 31 Publications. Collapse Publications.

Publications using 3074-TH	Applications	Species
Angabo, S;Pandi, K;David, K;Steinmetz, O;Makkawi, H;Farhat, M;Eli-Berchoer, L;Darawshi, N;Kawasaki, H;Nussbaum, G; CD47 and thrombospondin-1 contribute to immune evasion by Porphyromonas gingivalis Proceedings of the National Academy of Sciences of the United States of America 2024-11-19 [PMID: 39536084] (Bioassay, Human)	Bioassay	Human
Moniot, A;Schneider, C;Chardin, L;Yaniz-Galende, E;Genestie, C;Etiennot, M;Henry, A;Drelon, C;Le Formal, A;Langlois, B;Venat, L;Louvet, C;Favier, L;Lortholary, A;Berton-Rigaud, D;Dohollou, N;Desauw, C;Fabbro, M;Malaurie, E;Dubot, C;Kurtz, JE;Bonichon Lamichhane, N;Pujade-Lauraine, É;Jeanne, A;Leary, A;Dedieu, S; The CD47/TSP-1 axis: a promising avenue for ovarian cancer treatment and biomarker research Molecular cancer 2024-08-14 [PMID: 39138571] (Bioassay, Human)	Bioassay	Human
Wilson, ZS;Raya-Sandino, A;Miranda, J;Fan, S;Brazil, JC;Quiros, M;Garcia-Hernandez, V;Liu, Q;Kim, CH;Hankenson, KD;Nusrat, A;Parkos, CA; Critical role of thrombospondin-1 in promoting intestinal mucosal wound repair JCI insight 2024-07-30 [PMID: 39078701] (Bioassay, Human)	Bioassay	Human
Corbella, E;Fara, C;Covarelli, F;Porreca, V;Palmisano, B;Mignogna, G;Corsi, A;Riminucci, M;Maras, B;Mancone, C; THBS1 and THBS2 Enhance the In Vitro Proliferation, Adhesion, Migration and Invasion of Intrahepatic Cholangiocarcinoma Cells International journal of molecular sciences 2024-02-01 [PMID: 38339060] (Bioassay, Human)	Bioassay	Human
Farge, T;Nakhle, J;Lagarde, D;Cognet, G;Polley, N;Castellano, R;Nicolau, ML;Bosc, C;Sabatier, M;Sahal, A;Saland, E;Jeanson, Y;Guiraud, N;Boet, E;Bergoglio, C;Gotanègre, M;Mouchel, PL;Stuani, L;Larrue, C;Sallese, M;De Mas, V;Moro, C;Dray, C;Collette, Y;Raymond-Letron, I;Ader, I;Recher, C;Sarry, JE;Cabon, F;Vergez, F;Carriere, A; CD36 drives metastasis and relapse in acute myeloid leukemia Cancer research 2023-06-16 [PMID: 37327406] (Bioassay, Human)	Bioassay	Human
Krishna, S;Choudhury, A;Keough, MB;Seo, K;Ni, L;Kakaizada, S;Lee, A;Aabedi, A;Popova, G;Lipkin, B;Cao, C;Nava Gonzales, C;Sudharshan, R;Egladyous, A;Almeida, N;Zhang, Y;Molinaro, AM;Venkatesh, HS;Daniel, AGS;Shamardani, K;Hyer, J;Chang, EF;Findlay, A;Phillips, JJ;Nagarajan, S;Raleigh, DR;Brang, D;Monje, M;Hervey-Jumper, SL; Glioblastoma remodelling of human neural circuits decreases survival Nature 2023-05-03 [PMID: 37138086] (Bioassay, Human)	Bioassay	Human
N Ludwig, SS Yerneni, M Harasymczu, MJ Szczepa?sk, A G?uszko, W Kukwa, T Jordan, G Spanier, J Taxis, S Spoerl, JK Meier, CS Hinck, PG Campbell, TE Reichert, AP Hinck, TL Whiteside TGFbeta carrying exosomes in plasma: potential biomarkers of cancer progression in patients with head and neck squamous cell carcinoma British Journal of Cancer, 2023-02-21;0(0):. 2023-02-21 [PMID: 36810911] (Bioassay, Human)	Bioassay	Human
A Dasgupta, HT Ngo, D Tschoerner, N Touret, B da Rocha-A, K Jaqaman Multiscale imaging and quantitative analysis of plasma membrane protein-cortical actin interplay bioRxiv : the preprint server for biology, 2023-01-23;0(0):. 2023-01-23 [PMID: 36747866] (Bioassay, Human)	Bioassay	Human
S Touhami, F Béguier, T Yang, S Augustin, C Roubeix, F Blond, JB Conart, JA Sahel, B Bodaghi, C Delarasse, X Guillonnea, F Sennlaub Hypoxia Inhibits Subretinal Inflammation Resolution Thrombospondin-1 Dependently International Journal of Molecular Sciences, 2022-01-08;23(2):. 2022-01-08 [PMID: 35054863] (In Vivo, Mouse)	In Vivo	Mouse
M Shibuya, H Matsui, T Sasagawa, T Nagamatsu A simple detection method for the serum sFLT1 protein in preeclampsia Scientific Reports, 2021-10-18;11(1):20613. 2021-10-18 [PMID: 34663835] (ELISA Standard, Human)	ELISA Standard	Human
M Yao, J Sturdivant, A Ebrahimi, S Ganguly, T Elbayoumi Novel Pharmaceutical Strategy for Selective Abrogation of TSP1-Induced Vascular Dysfunction by Decoy Recombinant CD47 Soluble Receptor in Prophylaxis and Treatment Models Biomedicines, 2021-06-03;9(6):. 2021-06-03 [PMID: 34205047] (Bioassay, Mouse)	Bioassay	Mouse
S Lie, BR Vieira, S Arruda, M Simões, LM Ashander, JM Furtado, JR Smith Molecular Basis of The Retinal Pigment Epithelial Changes That Characterize The Ocular Lesion in Toxoplasmosis Microorganisms, 2019-09-29;7(10):. 2019-09-29 [PMID: 31569536] (Bioassay, Human)	Bioassay	Human
J Guillon, C Petit, M Moreau, B Toutain, C Henry, H Roché, N Bonichon-L, JP Salmon, J Lemonnier, M Campone, V Verrièle, E Lelièvre, C Guette, O Coqueret Regulation of senescence escape by TSP1 and CD47 following chemotherapy treatment Cell Death Dis, 2019-02-27;10(3):199. 2019-02-27 [PMID: 30814491] (Bioassay, Human)	Bioassay	Human
A Ojha, A Bhasym, S Mukherjee, GK Annarapu, T Bhakuni, I Akbar, T Seth, NK Vikram, S Vrati, A Basu, S Bhattachar, P Guchhait Platelet factor 4 promotes rapid replication and propagation of Dengue and Japanese encephalitis viruses EBioMedicine, 2018-12-05;39(0):332-347. 2018-12-05 [PMID: 30527622] (Bioassay, Human)	Bioassay	Human
G Inana, C Murat, W An, X Yao, IR Harris, J Cao RPE phagocytic function declines in age-related macular degeneration and is rescued by human umbilical tissue derived cells J Transl Med, 2018-03-13;16(1):63. 2018-03-13 [PMID: 29534722] (Bioassay, Human)	Bioassay	Human
Dong Hyun Kim, Hoon Lim, Dahm Lee, Soo Jin Choi, Wonil Oh, Yoon Sun Yang, Jeong Su Oh, Hyun Ho Hwang, Hong Bae Jeon Thrombospondin-1 secreted by human umbilical cord blood-derived mesenchymal stem cells rescues neurons from synaptic dysfunction in Alzheimer's disease model. Scientific Reports, 2018-01-10;0(0):2045-2322. 2018-01-10 [PMID: 29321508] (Bioassay, Mouse)	Bioassay	Mouse
R Bibes, S Gobron, F Vincent, C Mélin, N Vedrenne, A Perraud, F Labrousse, MO Jauberteau, F Lalloué SCO-spondin oligopeptide inhibits angiogenesis in glioblastoma Oncotarget, 2017-09-12;8(49):85969-85983. 2017-09-12 [PMID: 29156770] (Bioassay, Human)	Bioassay	Human
K Endt, J Goepfert, A Omlin, A Athanasiou, P Tennstedt, A Guenther, M Rainisio, DS Engeler, T Steuber, S Gillessen, T Joos, R Schiess Development and clinical testing of individual immunoassays for the quantification of serum glycoproteins to diagnose prostate cancer PLoS ONE, 2017-08-02;12(8):e0181557. 2017-08-02 [PMID: 28767721] (ELISA (Standard), Human)	ELISA (Standard)	Human
C Sallon, I Callebaut, I Boulay, J Fontaine, D Logeart-Av, C Henriquet, M Pugnière, X Cayla, P Monget, G Harichaux, V Labas, S Canepa, C Taragnat Thrombospondin-1 (TSP-1), a new bone morphogenetic protein-2 and -4 (BMP-2/4) antagonist identified in pituitary cells J. Biol. Chem., 2017-07-26;0(0):. 2017-07-26 [PMID: 28747434] (Bioassay, Enzyme Assay, Human)	Bioassay, Enzyme Assay	Human
J Zhai, J Liu, X Cheng, S Li, Y Hong, K Sun, ZJ Chen, Y Du, W Li Zinc finger gene 217 (ZNF217) Promoted Ovarian Hyperstimulation Syndrome (OHSS) through Regulating E2 Synthesis and Inhibiting Thrombospondin-1 (TSP-1) Sci Rep, 2017-06-12;7(1):3245. 2017-06-12 [PMID: 28607476] (Bioassay, Human)	Bioassay	Human
A Torres, MA Gubbiotti, RV Iozzo Decorin-inducible Peg3 Evokes Beclin 1-mediated Autophagy and Thrombospondin 1-mediated Angiostasis J. Biol. Chem, 2017-02-07;292(12):5055-5069. 2017-02-07 [PMID: 28174297] (Bioassay, Human)	Bioassay	Human
Senescent peritoneal mesothelium creates a niche for ovarian cancer metastases Cell Death Dis, 2016-12-29;7(12):e2565. 2016-12-29 [PMID: 28032864] (Bioassay, Human)	Bioassay	Human
The structural basis for CD36 binding by the malaria parasite Nat Commun, 2016-09-26;7(0):12837. 2016-09-26 [PMID: 27667267] (Enzyme Assay, Human)	Enzyme Assay	Human
The Different Binding Properties of Cultured Human Corneal Endothelial Cell Subpopulations to Descemet's Membrane Components Invest Ophthalmol Vis Sci, 2016-09-01;57(11):4599-605. 2016-09-01 [PMID: 27598865] (Bioassay, Human)	Bioassay	Human
Thrombospondin 1 Deficiency Ameliorates the Development of Adriamycin-Induced Proteinuric Kidney Disease PLoS ONE, 2016-05-19;11(5):e0156144. 2016-05-19 [PMID: 27196103] (Bioassay, Human)	Bioassay	Human
Zheng Y, Zou F, Wang J, Yin G, Le V, Fei Z, Liu J Photodynamic therapy-mediated cancer vaccination enhances stem-like phenotype and immune escape, which can be blocked by thrombospondin-1 signaling through CD47 receptor protein. J Biol Chem, 2015-02-19;290(14):8975-86. 2015-02-19 [PMID: 25697354] (Bioassay, Human)	Bioassay	Human
Sugita S, Kamao H, Iwasaki Y, Okamoto S, Hashiguchi T, Iseki K, Hayashi N, Mandai M, Takahashi M Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells. Invest Ophthalmol Vis Sci, 2015-01-20;56(2):1051-62. 2015-01-20 [PMID: 25604685] (Bioassay, Human)	Bioassay	Human
Cursiefen C, Maruyama K, Bock F, Saban D, Sadrai Z, Lawler J, Dana R, Masli S Thrombospondin 1 inhibits inflammatory lymphangiogenesis by CD36 ligation on monocytes. J. Exp. Med., 2011-05-02;208(5):1083-92. 2011-05-02 [PMID: 21536744] (In Vivo, Mouse)	In Vivo	Mouse
Peterson JE, Zurakowski D, Italiano JE Normal ranges of angiogenesis regulatory proteins in human platelets. Am. J. Hematol., 2010-07-01;85(7):487-93. 2010-07-01 [PMID: 20575035] (ELISA (Standard))	ELISA (Standard)
Lee YJ, Koch M, Karl D Variable inhibition of thrombospondin 1 against liver and lung metastases through differential activation of metalloproteinase ADAMTS1. Cancer Res., 2010-01-26;70(3):948-56. 2010-01-26 [PMID: 20103648] (Bioassay, Mouse)	Bioassay	Mouse
Estrogen regulation of thrombospondin-1 in human breast cancer cells. Int. J. Cancer, 2009-09-01;125(0):1045. 2009-09-01 [PMID: 19391135] (Bioassay, Human)	Bioassay	Human
Show All 31 Publications. Collapse Publications.

Array 3074-TH

• Thrombospondin-1 Antibodies
• Thrombospondin-1 ELISA Kits
• Thrombospondin-1 Lysate
• Thrombospondin-1 Protein
• Thrombospondin-1 ELISAs
• Thrombospondin-1 Proteins and Enzymes