Home » R-Spondin 1 » R-Spondin 1 Proteins » Recombinant Human R-Spondin 1 Biotinylated Protein, CF
Novus Biologicals products are now on bio-techne.com

Recombinant Human R-Spondin 1 Biotinylated Protein, CF

 Submit a Review

Images

 
(
Enlarge)
When Recombinant Human Lgr5/GPR49 Fc Chimera (8078-GP) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human R-Spondin 1 Protein (Catalog # BT4645B) binds with an ED50 of 4.50-54.0 ng/mL.
When Recombinant Human Lgr5/GPR49 Fc Chimera (8078-GP) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human R-Spondin 1 Protein (Catalog # BT4645B) binds with an ED50 of 4.50-54.0 ng/mL.
2 μg/lane of Biotinylated Recombinant Human R‑Spondin 1 Protein (Catalog # BT4645B) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BT4645-025/CF
Biotin
BT4645-025
Unconjugated
BT4645-025
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
BT4645-025
Carrier Free
BT4645-025/CF
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Human R-Spondin 1 Biotinylated Protein, CF Summary

Additional Information
Biotinylated
Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human Lgr5/GPR49 Fc Chimera (Catalog # 8078-GP) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human R-Spondin 1 (Catalog # BT4645B) binds with an ED50 of 4.50-54.0 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 1 protein
Ser21-Ala263
Accession #
N-terminal Sequence
Ser21 & Arg31
Structure / Form
Biotinylated via amines
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
25.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
29-42 kDa, under reducing conditions.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human R-Spondin 1 Biotinylated Protein, CF

  • Cristin 3
  • CRISTIN3
  • FLJ40906Roof plate-specific spondin-1
  • HRspo1
  • roof plate-specific spondin
  • RSPO
  • RSPO1
  • RSpondin 1
  • R-Spondin 1
  • R-spondin homolog (Xenopus laevis)
  • RSPONDIN
  • R-spondin1
  • R-spondin-1

Background

R-Spondin 1 (RSPO1), also known as Cristin 3, is a 27 kDa secreted protein in the R-Spondin family of Wnt/ beta -catenin signaling regulators (1). These proteins contain two adjacent cysteine-rich furin-like domains followed by a thrombospondin (TSP-1) motif and a region rich in basic residues. Mature human R-Spondin 1 shares 87% amino acid sequence identity with mouse and rat R-Spondin 1 (2). Alternative splicing generates additional isoforms that have a substituted N-terminus or lack the TSP-1 domain. R-Spondin 1 enhances canonical Wnt/ beta -catenin signaling by competing with the Wnt antagonist Dkk-1, binding to Frizzled-8, Kremen, LRP-6, Lgr4, Lgr5, and Lgr6, and enhancing cell surface availability of Wnt receptors (3-11). R-Spondin 1 functions in dorsal neural tube development (12) as well as male and female germ cell development (7, 10, 13). It also induces bone formation (6), intestinal crypt cell proliferation (14), angiogenesis (7, 15), and insulin secretion from pancreatic beta cells (11). Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids (16). Over the last several years, the understanding of the regulatory mechanisms and functional roles of RSPOs in many biological contexts has increased. Particularly, because a leucine-rich repeat containing G protein-coupled receptor 5 (LGR5), a stem cell marker originally identified as a marker for intestinal stem cells, and two closely related proteins, LGR4 and LGR6, were identified as cognate receptors for RSPOs, significant research progress has been made in understanding the functional roles of RSPO/LGR signaling in stem cell biology (17).
  1. Jin, Y.R. and J.K. Yoon (2012) Int. J. Biochem. Cell Biol. 44:2278.
  2. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
  3. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
  4. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
  5. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
  6. Kronke, G. et al. (2010) Arthritis Rheum. 62:2303.
  7. Caruso, M. et al. (2015) PLoS One 10:e0124213.
  8. Hao, H.-X. et al. (2012) Nature 485:195.
  9. de Lau, W. et al. (2011) Nature 476:293.
  10. Chassot, A.-A. et al. (2011) PLoS One 6:e25641.
  11. Wong, V.S.C et al. (2010) J. Biol. Chem. 285:21292.
  12. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
  13. Chassost, A.-A. et al. (2012) Development 139:4461.
  14. Kim, K.-A. et al. (2005) Science 309:1256.
  15. Gore, A.V. et al. (2011) Development 138:4875.
  16. Drost and Clevers. (2018) Nature Reviews Cancer 18:407.
  17. Raslan, H. et al. (2019) J. Biochem. Biocell. 106:26-34.

Customers Who Viewed This Item Also Viewed...

Wnt-4 Antibody
AF475
Wnt-4 Antibody
(1 Review)
(12 Publications)
Species: Mu
Applications: IHC, WB
SRY Antibody (1G4)
- BSA Free
NBP2-37463
ELISA SRY Antibody (1G4) - BSA Free
Western Blot SRY Antibody (1G4) - BSA Free
Western Blot SRY Antibody (1G4) - BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IHC, IHC-P, WB
beta-Catenin
Antibody [Unconjugate
AF1329
Western Blot beta-Catenin Antibody [Unconjugated]
Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]
Simple Western beta-Catenin Antibody [Unconjugated]
(2 Reviews)
(33 Publications)
Species: Hu, Mu, Rt
Applications: ChIP, CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
SOX9 Antibody
[Unconjugated]
AF3075
Immunocytochemistry SOX9 Antibody [Unconjugated]
Western Blot SOX9 Antibody [Unconjugated]
Immunocytochemistry SOX9 Antibody [Unconjugated]
(2 Reviews)
(154 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
FOXL2 Antibody
NB100-1277
Western Blot FOXL2 Antibody
Immunohistochemistry-Paraffin FOXL2 Antibody
Immunohistochemistry-Paraffin FOXL2 Antibody
(1 Review)
(72 Publications)
Species: Bv, Ma, Hu, Mu
Applications: ChIP, ICC/IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, PEP-ELISA, WB

SF-1/NR5A1/Steroidogen ...
NBP1-52823
Immunohistochemistry-Paraffin SF-1/NR5A1/Steroidogenic Factor 1 Antibody
Western Blot SF-1/NR5A1/Steroidogenic Factor 1 Antibody
(4 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
Dkk-1
[Unconjugated]
5439-DK
Cell Culture Dkk-1 [Unconjugated]
Cell Culture Dkk-1 [Unconjugated]
Bioactivity Dkk-1 [Unconjugated]
(90 Publications)
Species: Hu
Applications: BA
FGF-9
[Unconjugated]
273-F9
Bioactivity FGF-9 [Unconjugated]
(47 Publications)
Species: Hu
Applications: BA
DAX1/NR0B1
Antibody (H7431)
PP-H7431-00
DAX1/NR0B1 Antibody (H7431)
(2 Publications)
Species: Hu
Applications: WB
R-Spondin 2
[Unconjugated]
3266-RS
SDS-PAGE R-Spondin 2 [Unconjugated]
Bioactivity R-Spondin 2 [Unconjugated]
(19 Publications)
Species: Hu
Applications: BA
Lgr5/GPR49
Antibody - BSA Free
NBP1-28904
Immunohistochemistry Lgr5/GPR49 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Lgr5/GPR49 Antibody - BSA Free
Immunohistochemistry Lgr5/GPR49 Antibody - BSA Free
(1 Review)
(12 Publications)
Species: Hu, Mu, Po
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
LRP-6 Antibody
(255302) [Unconjugate
MAB1505
Flow Cytometry LRP-6 Antibody (255302) [Unconjugated]
Immunocytochemistry LRP-6 Antibody (255302) [Unconjugated]
(6 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, ICC
R-Spondin 4
[Unconjugated]
4575-RS
R-Spondin 4 [Unconjugated]
(8 Publications)
Species: Hu
Applications: BA
Kremen-1 Antibody
AF1647
Western Blot Kremen-1 Antibody
(2 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
Splicing Factor 1
Antibody (2E12)
H00007536-M01
Western Blot Splicing Factor 1 Antibody (2E12)
Western Blot Splicing Factor 1 Antibody (2E12)
Immunocytochemistry/ Immunofluorescence Splicing Factor 1 Antibody (2E12)
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
WT1 Antibody
(6F-H2) - BSA Free
NB110-60011
Western Blot WT1 Antibody (6F-H2) - BSA Free
Immunohistochemistry-Paraffin WT1 Antibody (6F-H2) - BSA Free
Western Blot WT1 Antibody (6F-H2) - BSA Free
(3 Reviews)
(16 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
beta-Catenin
Antibody [Unconjugate
AF1329
Western Blot beta-Catenin Antibody [Unconjugated]
Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]
Simple Western beta-Catenin Antibody [Unconjugated]
(2 Reviews)
(33 Publications)
Species: Hu, Mu, Rt
Applications: ChIP, CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
Wnt-3a
[Unconjugated]
5036-WN
Bioactivity Wnt-3a [Unconjugated]
Bioactivity Wnt-3a [Unconjugated]
Cell Culture Wnt-3a [Unconjugated]
(369 Publications)
Species: Hu
Applications: BA, BA

Publications for R-Spondin 1 (BT4645B) (0)

There are no publications for R-Spondin 1 (BT4645B).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for R-Spondin 1 (BT4645B) (0)

There are no reviews for R-Spondin 1 (BT4645B). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for R-Spondin 1 (BT4645B) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional R-Spondin 1 Products

Blogs on R-Spondin 1

There are no specific blogs for R-Spondin 1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

SOX9 was detected in immersion fixed BG01V human embryonic stem cells differentiated into early proximal lung progenitor cells using Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
SOX9 Antibody [Unconjugated]
AF3075

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human R-Spondin 1 Biotinylated Protein, CF and receive a gift card or discount.


Bioinformatics

Uniprot