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Recombinant Human Mesothelin (aa 35-289) His-tag Protein, CF

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2 μg/lane of Recombinant Human Mesothelin N-Terminal (aa 35-289) His-tag (Catalog# 10093-MS) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 27-39 kDa.
2 μg/lane of Recombinant Human Mesothelin N-Terminal (aa 35-289) His-tag (Catalog# 10093-MS) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Mesothelin (aa 35-289) His-tag Protein, CF Summary

Additional Information
N-Terminal/MPF
Details of Functionality
Bioassay data are not available.
Source
Mouse myeloma cell line, NS0-derived human Mesothelin protein
Arg35-Leu289, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Arg35
Structure / Form
Monomer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
27-39 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Mesothelin (aa 35-289) His-tag Protein, CF

  • CAK1 antigen
  • CAK1
  • megakaryocyte potentiating factor
  • Mesothelin
  • MPF
  • MPFSMRP
  • MSLN
  • Pre-pro-megakaryocyte-potentiating factor
  • SMR
  • soluble MPF mesothelin related protein

Background

Megakaryocyte-Potentiating Factor (MPF), is derived from a 70 kDa Mesothelin precursor (1-3). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic furin cleavage site between aa 288-293. The cleavage releases soluble N-terminal MPF (aa 37-286) and C-terminal Mesothelin (aa 296-606) remains attached to the cell surface via a GPI linkage. (3, 4). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation (2, 5). This recombinant human MPF shares 56% sequence identity with mouse and rat MPF. Apart from its role as a megakaryocyte potentiating cytokine, MPF may have the potential to serve as a biomarker for detection and monitoring of malignant mesothelioma and related cancers (6).
  1. Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
  2. Kojima, T. et al. (1995) J. Biol. Chem. 270:21984.
  3. Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
  4. Onda, M. et al. (2006) Clin. Cancer Res. 12:4225.
  5. Yamaguchi, N. et al. (1994) J. Biol. Chem. 269:805.
  6. Raiko, I. et al. (2017) Biochem. Biophy. Res. Com. 486:526.

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Recombinant Human
Mesothelin (aa 35-289 ...
10093-MS
Recombinant Human Mesothelin (aa 35-289) His-tag Protein, CF
Species: Hu
Applications: Bioactivity

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