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Recombinant Human Ly-6G6F Fc Chimera Protein, CF

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Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect 1-10 μg/mL.
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect 1-10 μg/mL.
2 μg/lane of Recombinant Human Ly-6G6F Fc Chimera Protein (10513-L6) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Ly-6G6F Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect 1-10 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human Ly-6G6F protein
Human Ly-6G6F
(Ala17-Trp235)
Accession # Q5SQ64.2		IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Ala17
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
55-62 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Ly-6G6F Fc Chimera Protein, CF

  • C6orf21
  • G6f
  • locus G6D
  • LY6G6D
  • LY6G6F
  • Ly-6G6F
  • lymphocyte antigen 6 complex locus protein G6f
  • lymphocyte antigen 6 complex, locus G6F
  • NG32

Background

Lymphocyte antigen 6 complex locus protein G6f, also known as G6f, is a 32-kDa single pass type I membrane protein belonging to the Immunoglobulin Superfamily that is encoded in the MHC (1). The extracellular domain is 281 aa in length and contains one disulfide bond (C35 ↔ C106) and an N-linked glycosylation site at S88. The ECD of human Ly-6G6F shares 64.5% and 63% sequence identity with mouse and rat Ly-6G6F, respectively. The intracellular tail of Ly-6G6F is 40 amino acids in length and contains one tyrosine residue (Y281), which is phosphorylated after treatment of cells with pervanadate. Ly-6G6F may play a role in signal transduction pathways involved with Grb2 and Grb7, such as the Ras-MAP kinase pathway (1). Ly-6G6F tyrosine phosphorylation was also found to take place in response to collagen, although not in response to the G protein-coupled receptor agonists, thrombin and ADP. A global proteomics approach identifies Ly-6G6F as a novel phosphorylated signaling proteins in GPVI-activated platelets (2, 3).  Leukocytes mRNA levels of Ly-6G6F mainly involved in inflammation were significantly higher in patients carrying granulin mutations compared with asymptomatic carriers (4).
  1. De Vet, E.C. et al. (2003) Biochem. J. 375:207.
  2. Garcia, A. et al. (2006) Proteomics. 6:5332.
  3. Lewandrowski U. et al. (2006) Mol. Cell Proteomics 5:226.
  4. Milanesi E. et al. (2013) Neurobiol. Aging. 34:1837.

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