Home » Kallikrein 3/PSA » Kallikrein 3/PSA Proteins » Recombinant Human Kallikrein 3/PSA Protein, CF
Novus Biologicals products are now on bio-techne.com

Recombinant Human Kallikrein 3/PSA Protein, CF

 (8 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Recombinant Human Kallikrein 3 (Catalog # 1344-SE) is measured byits ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide(Suc-RPY-pNA).
Recombinant Human Kallikrein 3 (Catalog # 1344-SE) is measured byits ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide(Suc-RPY-pNA).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Human Kallikrein 3/PSA Protein, CF Summary

Details of Functionality
Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA). The specific activity is >70 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human Kallikrein 3/PSA protein
Ala18-Pro261, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Ala18
Structure / Form
Pro form
Protein/Peptide Type
Recombinant Enzymes
Gene
KLK3
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Enzyme Activity
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
36 kDa, reducing conditions
Publications
Read Publications using
1344-SE in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in 50 mM Tris, 10 mM CaCl2,150 mM NaCl and 0.05% (w/v) Brij-35, pH 7.5.
Assay Procedure
  • Activation Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35, pH 7.5 (TCNB)
  • Assay Buffer: 50 mM Tris, 1 M NaCl, pH 8.0
  • Recombinant Human Kallikrein 3/PSA (rhKLK3) (Catalog # 1344-SE)
  • Bacterial Thermolysin (Thermolysin) (Catalog # 3097-ZN)
  • 1,10 Phenanthroline (Sigma, Catalog # 320056), 0.6 M stock in DMSO
  • Substrate: Suc-Arg-Pro-Tyr-pNa (AnaSpec, Catalog # 20586), 10 mM in deionized water
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rhKLK3 to 200 µg/mL in Activation Buffer.
  2. Dilute Thermolysin to 2 µg/mL in Activation Buffer.
  3. Combine equal volumes of 200 µg/mL rhKLK3 and 2 µg/mL Thermolysin.
  4. Incubate at 37 °C for 5 minutes.
  5. Dilute 1,10 Phenanthroline to 20 mM in Assay Buffer.
  6. Stop Thermolysin activity  by adding 1,10 Phenanthroline to a final concentration of 10 mM.
  7. Dilute activated rhKLK3 to 20 ng/µL in Assay Buffer.
  8. Dilute Substrate to 2 mM in Assay Buffer.
  9. Load 50 µL of the 20 ng/µL rhKLK3 in a plate and start the reaction by adding 50 µL of 2 mM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 2 mM Substrate.
  10. Read at a wavelength of 405 nm (bottom read) in kinetic mode for 5 minutes.
  11. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/mol
ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg)

     *Adjusted for Substrate Blank 
     **Using the extinction coefficient 8800 M-1cm-1 
     ***Using the path correction 0.32 cm
     Note: the output of many spectrophotometers is in mOD. Per Well:
  • rhKLK3: 1 µg
  • Substrate: 1 mM

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Kallikrein 3/PSA Protein, CF

  • APS seminin
  • EC 3.4.21
  • EC 3.4.21.77
  • Gamma-seminoprotein
  • hK3
  • Kallikrein 3
  • kallikrein 3, (prostate specific antigen)
  • Kallikrein-3
  • kallikrein-related peptidase 3
  • KLK2A1
  • KLK3
  • P-30 antigen
  • prostate specific antigen
  • prostate-specific antigen
  • PSA semenogelase
  • PSA
  • Semenogelase
  • Seminin

Background

Kallikrein 3, commonly known as prostate specific antigen (PSA), is a serine protease of the human tissue Kallikrein gene family (1). PSA is synthesized in the ductal and acinar epithelium of the prostate gland and secreted into the seminal plasma in high concentrations (0.5 - 2 g/L) (2). A small portion of PSA “leaks” into the systemic circulation, the levels of which increase significantly (30-fold) from prostate cancer tissue than normal prostate tissue (3). PSA has become a well established tumor marker that aids the diagnosis, staging, and follow up of prostate cancer.

The deduced amino acid sequence of human PSA consists of a signal peptide, a short pro region and a mature/active enzyme. The pro-enzyme is activated, possibly by active Kallikreins 2, 4 or 15 in vivo (4). rhPSA is activated by thermolysin, a zinc protease. The active PSA cleaves several tyrosyl peptide bonds in semenogelins I and II, which are the major gel-forming proteins produced by the seminal vesicles (5). Several inhibitors including serpin A3/ alpha 1-antichymotrypsin (ACT) and alpha 2‑macroglobulin are known to form complexes with PSA.

  1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  2. Ward, A.M. et al. (2001) Ann. Clin. Biochem. 38:633.
  3. Jain, S. et al. (2002) Postgrad. Med. J. 78:646.
  4. LiLja H. (2003) Urology 62:27.
  5. Takayama, T.K. et al. (1997) J. Biol. Chem. 272:21582.

Customers Who Viewed This Item Also Viewed...

Androgen R/NR3C4
[p Ser213, p Ser210] ...
NB100-56603
Western Blot Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free
(14 Publications)
Species: Ca, Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB
Prostatic Acid
Phosphatase/ACPP Anti ...
AF6240
Western Blot Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]
Immunohistochemistry Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]
ELISA Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]
(1 Publication)
Species: Hu
Applications: ELISA, IHC, WB
AKR1B1 Antibody
NBP1-89146
Immunohistochemistry-Paraffin AKR1B1 Antibody
Immunohistochemistry-Paraffin AKR1B1 Antibody
Immunohistochemistry-Paraffin AKR1B1 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Amphiregulin
Antibody
AF262
Neutralization Amphiregulin Antibody
Immunocytochemistry Amphiregulin Antibody
Immunohistochemistry Amphiregulin Antibody
(1 Review)
(35 Publications)
Species: Hu
Applications: ICC, IHC, Neut, WB
Ferredoxin
Reductase Antibody
NBP2-38530
Western Blot Ferredoxin Reductase Antibody
Immunohistochemistry-Paraffin Ferredoxin Reductase Antibody
Immunohistochemistry-Paraffin Ferredoxin Reductase Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Adenine Nucleotide
Translocator 2 Antibo ...
NBP2-92630
Immunocytochemistry/ Immunofluorescence Adenine Nucleotide Translocator 2 Antibody - Azide and BSA Free
Immunohistochemistry-Paraffin Adenine Nucleotide Translocator 2 Antibody - Azide and BSA Free
Western Blot Adenine Nucleotide Translocator 2 Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
ASAP1 Antibody
NBP2-48909
Immunohistochemistry-Paraffin ASAP1 Antibody
Knockout Validated ASAP1 Antibody
Immunohistochemistry ASAP1 Antibody
(2 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KO
Reg3A Antibody
NBP2-24763
Western Blot Reg3A Antibody
Immunohistochemistry-Paraffin Reg3A Antibody
(3 Publications)
Species: Hu, Pm
Applications: IHC, IHC-P, WB
PAG3 Antibody
NBP2-24614
Western Blot PAG3 Antibody
Species: Hu, Mu, Ma-Op, Pm, Rt
Applications: WB
PAPOLA Antibody
NBP1-87345
Immunohistochemistry-Paraffin PAPOLA Antibody
Immunocytochemistry/ Immunofluorescence PAPOLA Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
MRPS30 Antibody
NBP1-87343
Immunohistochemistry-Paraffin MRPS30 Antibody
Immunohistochemistry-Paraffin MRPS30 Antibody
Immunohistochemistry-Paraffin MRPS30 Antibody
Species: Hu, Rt
Applications: IHC, IHC-P, WB
TUSC2 Antibody -
BSA Free
NBP3-04411
Immunohistochemistry-Paraffin TUSC2 Antibody - BSA Free
Immunohistochemistry-Paraffin TUSC2 Antibody - BSA Free
Immunohistochemistry-Paraffin TUSC2 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
PDAP1 Antibody
NBP3-20223
Western Blot PDAP1 Antibody
Immunoprecipitation PDAP1 Antibody
Immunocytochemistry/ Immunofluorescence PDAP1 Antibody
Species: Hu
Applications: ICC/IF, IP, WB

PSMA/FOLH1/NAALADase I ...
NBP1-45057
Western Blot PSMA/FOLH1/NAALADase I Antibody (GCP-04) - BSA Free
Immunohistochemistry PSMA/FOLH1/NAALADase I Antibody (GCP-04) - BSA Free
Immunohistochemistry PSMA/FOLH1/NAALADase I Antibody (GCP-04) - BSA Free
(2 Reviews)
(10 Publications)
Species: Ca, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Kallikrein 2
Antibody
NBP1-06046
Western Blot Kallikrein 2 Antibody
Immunohistochemistry-Paraffin Kallikrein 2 Antibody
Immunohistochemistry-Paraffin Kallikrein 2 Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
ACAT1 Antibody
NBP1-89284
Western Blot ACAT1 Antibody
Immunocytochemistry/ Immunofluorescence ACAT1 Antibody
Immunohistochemistry-Paraffin ACAT1 Antibody
(1 Review)
(5 Publications)
Species: Hu, Po
Applications: ICC/IF, IHC, IHC-P, KD, WB
POMT1 Antibody
NBP3-12487
Immunohistochemistry-Paraffin POMT1 Antibody
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
APC Antibody - BSA
Free
NB100-91662
Immunocytochemistry/ Immunofluorescence APC Antibody - BSA Free
Flow Cytometry APC Antibody - BSA Free
Immunohistochemistry-Paraffin APC Antibody - BSA Free
(2 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(664 Publications)
Species: Mu
Applications: ELISA
Recombinant Human
Kallikrein 3/PSA Prot ...
1344-SE
Recombinant Human Kallikrein 3/PSA Protein, CF
Species: Hu
Applications: Enzyme Activity

Publications for Kallikrein 3/PSA (1344-SE)(8)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 3 applications: Bioassay, ELISA Standard, Enzyme Assay.


Filter By Application
Bioassay
(3)
ELISA Standard
(1)
Enzyme Assay
(2)
All Applications
Filter By Species
Human
(6)
N/A
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using 1344-SE Applications Species
Küper, D;Klos, J;Kühl, F;Attili, R;Brand, K;Weissenborn, K;Lichtinghagen, R;Huber, R; Influence of Anticoagulants and Heparin Contaminants on the Suitability of MMP-9 as a Blood-Derived Biomarker International journal of molecular sciences 2024-09-20 [PMID: 39337591] (Bioassay, Human) Bioassay Human
Saeed, MA;Peng, B;Kim, K;Rawat, K;Kuehm, LM;Siegel, ZR;Borkowski, A;Habib, N;Van Tine, B;Sheikh, N;Tuyen, V;Thorek, DLJ;Fehniger, TA;Pachynski, RK; High dimensional analyses reveal IL-15 enhances activation of Sipuleucel-T lymphocyte subsets and reverses immunoresistance Cancer immunology research 2024-02-26 [PMID: 38407894] (Bioassay, Human) Bioassay Human
Jin, Y;Huang, Z;Xu, B;Chen, J; Localization of multiple DNAzymes as a branchedzyme-powered nanodevice for the immunoassay of tumor biomarkers Analytica chimica acta 2023-09-15 [PMID: 37455088] (ELISA Standard, N/A) ELISA Standard N/A
CP Mao, SC Wang, YP Su, SH Tseng, L He, AA Wu, RBS Roden, J Xiao, CF Hung Protein detection in blood with single-molecule imaging Science Advances, 2021-08-11;7(33):. 2021-08-11 [PMID: 34380620] (Human) Human
Kantyka T, Fischer J, Wu Z, Declercq W, Reiss K, Schroder J, Meyer-Hoffert U Inhibition of kallikrein-related peptidases by the serine protease inhibitor of Kazal-type 6. Peptides, 2011-03-23;32(6):1187-92. 2011-03-23 [PMID: 21439340] (Human) Human
Gokarna A, Jin LH, Hwang JS, Cho YH, Lim YT, Chung BH, Youn SH, Choi DS, Lim JH Quantum dot-based protein micro- and nanoarrays for detection of prostate cancer biomarkers. Proteomics, 2008-05-01;8(9):1809-18. 2008-05-01 [PMID: 18442169] (Bioassay, Human) Bioassay Human
Mukai S, Fukushima T, Naka D, Tanaka H, Osada Y, Kataoka H Activation of hepatocyte growth factor activator zymogen (pro-HGFA) by human kallikrein 1-related peptidases. FEBS J., 2008-01-25;275(5):1003-17. 2008-01-25 [PMID: 18221492] (Enzyme Assay, Human) Enzyme Assay Human
Yoon H, Laxmikanthan G, Lee J, Blaber SI, Rodriguez A, Kogot JM, Scarisbrick IA, Blaber M Activation profiles and regulatory cascades of the human kallikrein-related peptidases. J. Biol. Chem., 2007-09-06;282(44):31852-64. 2007-09-06 [PMID: 17823117] (Enzyme Assay) Enzyme Assay

Reviews for Kallikrein 3/PSA (1344-SE) (0)

There are no reviews for Kallikrein 3/PSA (1344-SE). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Kallikrein 3/PSA (1344-SE) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Kallikrein 3/PSA Products

Blogs on Kallikrein 3/PSA

There are no specific blogs for Kallikrein 3/PSA, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Recombinant&nbsp;Human Amphiregulin (Catalog&nbsp;# 262&#8209;AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant&nbsp;Human Amphiregulin (50&nbsp;ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog&nbsp;# AF262). The ND50 is typically 0.3-1&nbsp;µg/mL.
Amphiregulin Antibody
AF262

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Kallikrein 3/PSA Protein, CF and receive a gift card or discount.


Bioinformatics

Gene Symbol KLK3
Uniprot