| Reactivity | HuSpecies Glossary |
| Applications | Bioactivity |
| Format | Carrier-Free |
| Details of Functionality | Measured by its binding ability in a functional ELISA. When Recombinant Human IL1RAPL2 His-tag (Catalog # 11207-MR) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human PTPRD Fc Chimera (Catalog # 9995-PR) binds with an ED50 of 45.0-450 ng/mL. |
| Source | Chinese Hamster Ovary cell line, CHO-derived human IL1RAPL2 protein Thr17-Glu356, with a C-terminal 6-His tag |
| Accession # | |
| N-terminal Sequence | Thr17 and Lys20 |
| Protein/Peptide Type | Recombinant Proteins |
| Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Endotoxin Note | <0.10 EU per 1 μg of the protein by the LAL method. |
| Dilutions |
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| Theoretical MW | 40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| SDS-PAGE | 40-60 kDa, under reducing conditions. |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Reconstitution Instructions | Reconstitute at 500 μg/mL in PBS. |
The Interleukin 1 receptor family (IL-1R) comprises at least eleven members including IL-1 RI (IL-1R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL-1 R3), ST2 (T1/IL‑1 R4), IL‑18 R alpha (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 R beta (AcPL/IL-1 R7), IL1RAPL1 (TIGIRR-2/IL-1 R8), and IL1RAPL2 (TIGIRR-1/IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain (1). IL-1 receptor accessory protein-like 2 (IL1RAPL2) is alternately known as IL-1 R9 and three immunoglobulin domain containing IL-1 receptor-related molecule 1 (TIGIRR-1) and is expressed in the brain (2). Its sequence predicts an 686 amino acid (aa) residue type I transmembrane glycoprotein with a 17 aa signal peptide, a 339 aa extracellular region containing three Ig-like domains, an 18 aa transmembrane domain and a 312 aa cytoplasmic tail (3). By comparison to other IL-1 receptor family proteins, IL1RAPL2 has a C-terminal cytoplasmic extension beyond the TIR domain that is found in IL-1RAPL1 and SIGIRR but not other family members (3). Human and mouse IL1RAPL2 share approximately 95% aa sequence identity. Human IL1RAPL2 is most homologous (63%) to IL-1RAPL1, a receptor protein that is highly expressed in hippocampus and is involved in X-linked mental retardation (4, 5). Genes for both have been localized to human chromosome Xq22. A ligand for IL1RAPL2 has not been identified (1).
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