Recombinant Human IGF-I/IGF-1, Animal-Free Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Insulin-like growth factor I (IGF-I or IGF-1), also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I/IGF-1  is synthesized as two precursor isoforms with N- and alternate C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I/IGF-1 is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature human IGF-I/IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-I/IGF-1, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF‑II/IGF-2. Circulating IGF-I/IGF-1 is produced by hepatocytes, while local IGF-I/IGF-1 is produced by many other tissues in which it has paracrine effects (1). IGF-I/IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I/IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I/IGF-1 binds IGF‑I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I/IGF-1 association with IGF binding proteins increases its plasma half‑life and modulates its interactions with receptors (10).

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

• All molecular biology procedures use animal-free media and dedicated labware.
• Dedicated fermentors are utilized in committed animal-free areas.

Purification

• Protein purification columns are animal-free.
• Bulk proteins are filtered using animal-free filters.
• Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

• Low Endotoxin Level.
• No impairment of biological activity.
• High quality product obtained under stringent conditions.
• For ex vivo research or bioproduction, additional documentation can be provided.

Please read our complete Animal-Free Statement

 

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.

Showing Publications 1 - 2 of 2.

Publications using AFL291	Applications	Species
R Sharma, A George, M Nimmagadda, D Ortolan, BS Karla, Z Qureshy, D Bose, R Dejene, G Liang, Q Wan, J Chang, BS Jha, O Memon, KJ Miyagishim, A Rising, M Lal, E Hanson, R King, MM Campos, M Ferrer, J Amaral, D McGaughey, K Bharti Epithelial phenotype restoring drugs suppress macular degeneration phenotypes in an iPSC model Nature Communications, 2021-12-15;12(1):7293. 2021-12-15 [PMID: 34911940] (Bioassay, Human)	Bioassay	Human
Q Zheng, H Gan, F Yang, Y Yao, F Hao, L Hong, L Jin Cytoplasmic m1A reader YTHDF3 inhibits trophoblast invasion by downregulation of m1A-methylated IGF1R Cell Discov, 2020-03-10;6(0):12. 2020-03-10 [PMID: 32194978] (Bioassay, Human)	Bioassay	Human

Array AFL291

• IGF-I/IGF-1 Antibodies
• IGF-I/IGF-1 ELISA Kit
• IGF-I/IGF-1 Lysates
• IGF-I/IGF-1 Proteins
• IGF-I/IGF-1 ELISAs
• IGF-I/IGF-1 Proteins and Enzymes