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Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein, CF

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1 μg per lane of Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460 (Catalog # SP-600) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.
1 μg per lane of Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460 (Catalog # SP-600) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein, CF Summary

Details of Functionality
This protein is useful as a substrate in in vitro assays using recombinant E3 Ubiquitin ligases and small molecule degraders. Reaction conditions will need to be optimized for each specific application.
Source
E. coli-derived human BRD4 protein
Glu49 - Glu460 with N-terminal 8-His and (DYKDDDDK) tags
Accession #
Protein/Peptide Type
Recombinant Proteins
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
SP-600 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein, CF

  • BRD4
  • bromodomain containing 4
  • bromodomain-containing 4
  • CAPchromosome-associated protein
  • HUNK1
  • HUNK1bromodomain-containing protein 4
  • HUNKI
  • MCAP
  • Protein HUNK1

Background

Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory.  BRD4 has two N-terminal bromodomains and one NET (N-terminal Extra Terminal) domain.  BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA Polymerase II.  BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others.  This recombinant protein contains an N-terminal portion of BRD4 including both bromodomains. This protein is useful as a substrate in in vitro assays using recombinant E3 Ubiquitin ligases and small molecule degraders.
  1. Devaiah, B.N. et al. (2012) Proc. Natl. Acad. Sci. 109:6927.
  2. Gadd, M.S. et al. (2017) Nat. Chem. Biol. 13:514.
  3. Wang, R. et al. (2012) J. Biol. Chem. 287:10738.
  4. Zhang, W. et al. (1994) J. Biol. Chem. 287:43137.

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Recombinant Human
His8-(DYKDDDDK)-BRD4 ...
SP-600
Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein, CF
Species: Hu
Applications: Bioactivity

Publications for BRD4 (SP-600)(1)

We have publications tested in 1 confirmed species: N/A.

We have publications tested in 1 application: Bioassay.


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