| Reactivity | HuSpecies Glossary |
| Format | Carrier-Free |
| Source | E. coli-derived human HSP40/DNAJB1 protein Met1 - Thr340 with a C-terminal 6-His tag |
| Accession # | |
| Protein/Peptide Type | Recombinant Proteins |
| Gene | DNAJB1 |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. |
| Theoretical MW | 39 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Supplied as a solution in HEPES, NaCl and TCEP. |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. |
Heat shock protein 40 (HSP40, also known as DNAJB1) is the human homologue of the bacterial DnaJ heat shock protein. Heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms, and they are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. HSP40 is a stress inducible chaperone that colocalizes with HSP70 and can bind unfolded proteins and prevent protein denaturation and aggregation. The conserved amino terminal J domain can interact with HSP70 and stimulate its ATPase activity. HSP40 and HSP70 are required to target some misfolded proteins to the Ubiquitin E3 ligase CHIP (Stub1) for subsequent ubiquitination.
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