I am interested in developing a surrogate ADCC assay (FC gamma III a binding assay) for which, I would require a CD16 Recombinant Protein as an antigen and Anti-Human IgG (Fc specific) as a secondary antibody as I am planning to perform this assay in an ELISA format. With this brief description, could you please suggest me the right CD16 Recombinant Protein among the three listed products in your web site (www.novusbio.com/proteins/cd16). Appreciate if you can share a reference article, where the use of this protein is cited.

Name: Recombinant Human Fc gamma RIIIA/CD16a (V176F) Protein, CF
Brand: Novus Biologicals
SKU: 8894-FC
Price: 423 USD

Question

Accepted Answer

The right CD16 protein for you depends on what sort of functionality you require for your assay. H00002214-P01 is made by a Taiwanese company called Abnova and we distribute it for them. They do not test the activity of their recombinant proteins. The proteins are produced in a cell-free wheat germ system with an N-terminal 26kDa GST-tag. Due to this expression system, in our experience, the proteins do not always undergo the same folding and post-transnational modifications they might undergo in an endogenous cell. Because of this, we do not recommend this protein for use in functional assays and do not guarantee them for this use. They are mainly intended for use in Western Blots and ELISA as a positive control with their antibody. NBP1-98908 is produced in E. coli and contains a His-tag and it also not guaranteed for functionality but may be due to its E. coli expression system. H00002214-G01 is also an Abnova and is made in their cell-free wheat germ expression system with proprietary liposome technology. This is a recently developed system by them and is intended to help the proteins fold and maintain functionality. Which protein would be best for you depends on what you require for your assay. Here is a link to our antibodies to the Fc region of human IgG. The best antibody for you will depend on what you would like to use for detection.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	Bioactivity
Format	Carrier-Free

Additional Information	Low-affinity V176F polymorphic variant
Details of Functionality	Measured by its binding ability in a functional ELISA. When Recombinant Human Fc gamma RIIIa/CD16a (V176F) was immobilize on a Ms x PolyHis coated plate, it binds biotinylated Human IgG. The concentration of biotinylated Human IgG that produces 50% of the optimal binding response is approximately 0.5-2.5 μg/mL.
Source	Human embryonic kidney cell, HEK293-derived human Fc gamma RIIIA/CD16a protein Gly17-Gln208, with a C-terminal 6-His tag
Accession #	P08637
N-terminal Sequence	Gly17 & Met18
Protein/Peptide Type	Recombinant Proteins
Gene	FCGR3A
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note	<0.10 EU per 1 μg of the protein by the LAL method.

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in sterile PBS.
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions	Reconstitute at 100 μg/mL in PBS.

Publications using 8894-FC	Applications	Species
Y Kitanaga, D Yamajuku, S Kubo, K Nakamura, M Maeda, M Seki, Y Kaneko, F Kinugasa, T Morokata, Y Kondo, H Yoshinari, S Nakayamada, T Sumida, Y Tanaka Discovery of a novel Igbeta and FcgammaRIIB cross-linking antibody, ASP2713, and its potential application in the treatment of systemic lupus erythematosus International immunopharmacology, 2021-11-12;101(0):108343. 2021-11-12 [PMID: 34781122] (ELISA Capture, Human)	ELISA Capture	Human
Zhang X, Olsen H, Chen S, So E, Zhou H, Burch E, Merigeon E, Block D, Strome S Anti-CD20 Antibody with Multimerized Fc Domains: A Novel Strategy To Deplete B Cells and Augment Treatment of Autoimmune Disease. J Immunol, 2015-12-22;196(3):1165-76. 2015-12-22 [PMID: 26695368] (Surface Plasmon Resonance)	Surface Plasmon Resonance

Recombinant Human Fc gamma RIIIA/CD16a (V176F) Protein, CF

Species Glossary

Images

Product Details

Order Details

Recombinant Human Fc gamma RIIIA/CD16a (V176F) Protein, CF Summary

Applications/Dilutions

Packaging, Storage & Formulations

Notes

Alternate Names for Recombinant Human Fc gamma RIIIA/CD16a (V176F) Protein, CF

Background

Customers Who Viewed This Item Also Viewed...

Publications for Fc gamma RIIIA/CD16a (8894-FC)(2)

Reviews for Fc gamma RIIIA/CD16a (8894-FC) (0)

FAQs for Fc gamma RIIIA/CD16a (8894-FC). (Showing 1 - 1 of 1 FAQs).

Additional Fc gamma RIIIA/CD16a Products

Blogs on Fc gamma RIIIA/CD16a

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics

Gene Symbol	FCGR3A
Uniprot	P08637()