Recombinant Human Ephrin-A5 Fc Chimera Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Ephrin‑A5, also known as AL‑1, RAGS, and LERK‑7, is an approximately 25 kDa member of the Ephrin‑A family of GPI‑anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin‑A ligands are structurally related to the extracellular domains of the transmembrane Ephrin‑B ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Ephrin‑A5 preferentially interacts with receptors in the EphA family but also with EphB2 (3). Mature human Ephrin‑A5 shares 99.5% aa sequence identity with mouse and rat Ephrin‑A5, respectively (4). Ephrin‑A5 is expressed in multiple tissues during development, particularly in the brain (5, 6). It can exert repulsive or attractive effects on migrating neurons in the developing brain and motor column of the spinal cord (6‑10). Ephrin‑A5 repels migrating axons by inducing growth cone collapse and neurite retraction and by inhibiting the neurotrophic effects of NGF and BDNF (3, 11, 12). It interacts in cis with EphA3 on retinal axon growth cones which reduces axonal sensitivity to Ephrin‑A5 in trans (13). In the adult, Ephrin‑A5 is expressed on hippocampal neurons and astrocytes and induces the development of hippocampal synapses (9, 14, 15). It supports the proliferation of neural progenitors and the survival of newly differentiated neurons (14). Ephrin‑A5 also functions as a tumor suppressor (16). Its normal function in inhibiting EGFR signaling is compromised by its down‑regulation in glioma (16). Ephrin‑A5 is also expressed by muscle precursor cells and interacts with EphA4 to restrict their migration to the correct locations during forelimb morphogenesis (17).

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chicken, Virus - Henipavirus.

We have publications tested in 7 applications: Bioassay, Coprecipitation Assay, ICC, IHC, IHC-Fr, Immunoprecipitation, In Vivo.

Showing Publications 1 - 10 of 37. Show All 37 Publications. Collapse Publications.

Publications using 374-EA	Applications	Species
Madasu, C;Liao, Z;Parks, SE;Sharma, KL;Bohren, KM;Ye, Q;Li, F;Palaniappan, M;Tan, Z;Yuan, F;Creighton, CJ;Tang, S;Masand, RP;Guan, X;Young, DW;Monsivais, D;Matzuk, MM; Identification of potent pan-ephrin receptor kinase inhibitors using DNA-encoded chemistry technology Proceedings of the National Academy of Sciences of the United States of America 2024-05-07 [PMID: 38701119] (Bioassay, Human)	Bioassay	Human
SL Banerjee, F Lessard, FJM Chartier, K Jacquet, AI Osornio-He, V Teyssier, K Ghani, N Lavoie, JN Lavoie, M Caruso, P Laprise, S Elowe, JP Lambert, N Bisson EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks Cell Reports, 2022-07-05;40(1):111031. 2022-07-05 [PMID: 35793621] (Bioassay, Human)	Bioassay	Human
C Cai, M Zhang, L Liu, H Zhang, Y Guo, T Lan, Y Xu, P Ma, S Li ADAM10-cleaved ephrin-A5 contributes to prostate cancer metastasis Cell Death & Disease, 2022-05-12;13(5):453. 2022-05-12 [PMID: 35551177] (Bioassay, Human)	Bioassay	Human
BA Creighton, S Afriyie, D Ajit, CR Casingal, KM Voos, J Reger, AM Burch, E Dyne, J Bay, JK Huang, ES Anton, MM Fu, DN Lorenzo Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A Elife, 2021-11-23;10(0):. 2021-11-23 [PMID: 34812142] (Bioassay, Human)	Bioassay	Human
L Teo, AG Boghdadi, J Homman-Lud, IC Mundinano, WC Kwan, JA Bourne Replicating infant-specific reactive astrocyte functions in the injured adult brain Progress in neurobiology, 2021-06-17;0(0):102108. 2021-06-17 [PMID: 34147584] (Bioassay, Human)	Bioassay	Human
TP Light, M Gomez-Sole, Z Wang, K Karl, E Zapata-Mer, MP Gehring, BC Lechtenber, TV Pogorelov, K Hristova, EB Pasquale A cancer mutation promotes EphA4 oligomerization and signaling by altering the conformation of the SAM domain The Journal of Biological Chemistry, 2021-06-15;297(1):100876. 2021-06-15 [PMID: 34139238] (Bioassay, Human)	Bioassay	Human
Mutually Repulsive EphA7–EfnA5 Organize Region-to-Region Corticopontine Projection by Inhibiting Collateral Extension [PMID: 33906900] (Bioassay, Mouse)	Bioassay	Mouse
J Jukonen, L Moyano-Gal, K Höpfner, EA Pietilä, L Lehtinen, K Huhtinen, E Gucciardo, J Hynninen, S Hietanen, S Grénman, PM Ojala, O Carpén, K Lehti Aggressive and recurrent ovarian cancers upregulate ephrinA5, a non-canonical effector of EphA2 signaling duality Scientific Reports, 2021-04-23;11(1):8856. 2021-04-23 [PMID: 33893375] (Bioassay, Human)	Bioassay	Human
YL Chen, YC Yen, CW Jang, SH Wang, HT Huang, CH Chen, JR Hsiao, JY Chang, YW Chen Ephrin A4-ephrin receptor A10 signaling promotes cell migration and spheroid formation by upregulating NANOG expression in oral squamous cell carcinoma cells Scientific Reports, 2021-01-12;11(1):644. 2021-01-12 [PMID: 33436772] (Human)		Human
T Verheyen, T Fang, D Lindenhofe, Y Wang, K Akopyan, A Lindqvist, B Högberg, AI Teixeira Spatial�organization-dependent EphA2 transcriptional responses revealed by ligand nanocalipers Nucleic Acids Res., 2020-06-04;0(0):. 2020-06-04 [PMID: 32352518] (Bioassay, Human)	Bioassay	Human
ED Laing, CK Navaratnar, S Cheliout D, SR Petzing, Y Xu, SL Sterling, GA Marsh, LF Wang, M Amaya, DB Nikolov, R Cattaneo, CC Broder, K Xu Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus Proc. Natl. Acad. Sci. U.S.A., 2019-09-23;0(0):. 2019-09-23 [PMID: 31548390] (Coprecipitation Assay, Virus - Henipavirus)	Coprecipitation Assay	Virus - Henipavirus
DM Habiel, MS Espindola, IC Jones, AL Coelho, B Stripp, CM Hogaboam CCR10+ epithelial cells from idiopathic pulmonary fibrosis lungs drive remodeling JCI Insight, 2018-08-23;3(16):. 2018-08-23 [PMID: 30135312] (Bioassay, Human)	Bioassay	Human
T Teng, A Gaillard, A Muzerelle, P Gaspar EphrinA5 Signaling Is Required for the Distinctive Targeting of Raphe Serotonin Neurons in the Forebrain eNeuro, 2017-02-06;4(1):. 2017-02-06 [PMID: 28197551] (Bioassay, Mouse)	Bioassay	Mouse
Patricia F Maness The Neural Cell Adhesion Molecule NCAM Promotes Clustering and Activation of EphA3 Receptors in GABAergic Interneurons to Induce RhoA/ROCK-mediated Growth Cone Collapse J. Biol. Chem., 2016-11-01;0(0):. 2016-11-01 [PMID: 27803162] (Bioassay, Mouse)	Bioassay	Mouse
Anks1a regulates COPII-mediated anterograde transport of receptor tyrosine kinases critical for tumorigenesis Nat Commun, 2016-09-13;7(0):12799. 2016-09-13 [PMID: 27619642] (Bioassay, Mouse)	Bioassay	Mouse
The extracellular interactome of the human adenovirus family reveals diverse strategies for immunomodulation Nat Commun, 2016-05-05;7(0):11473. 2016-05-05 [PMID: 27145901] (Bioassay, Human)	Bioassay	Human
K Gerstmann, D Pensold, J Symmank, M Khundadze, CA Hübner, J Bolz, G Zimmer Thalamic afferents influence cortical progenitors via ephrin A5-EphA4 interactions Development, 2014-12-05;142(1):140-50. 2014-12-05 [PMID: 25480914] (Bioassay, Mouse)	Bioassay	Mouse
Maury Y, Come J, Piskorowski R, Salah-Mohellibi N, Chevaleyre V, Peschanski M, Martinat C, Nedelec S Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes. Nat Biotechnol, 2014-11-10;33(1):89-96. 2014-11-10 [PMID: 25383599] (Bioassay, Human)	Bioassay	Human
Schaupp A, Sabet O, Dudanova I, Ponserre M, Bastiaens P, Klein R The composition of EphB2 clusters determines the strength in the cellular repulsion response. J Cell Biol, 2014-01-27;204(3):409-22. 2014-01-27 [PMID: 24469634] (Bioassay, Human)	Bioassay	Human
Szepietowska , Barbara, Horvath , Tamas L, Sherwin , Robert S Role of synaptic plasticity and EphA5-ephrinA5 interaction within the ventromedial hypothalamus in response to recurrent hypoglycemia. Diabetes, 2013-11-12;63(3):1140-7. 2013-11-12 [PMID: 24222347] (In Vivo, Rat)	In Vivo	Rat
Defourny J, Poirrier A, Lallemend F, Mateo Sanchez S, Neef J, Vanderhaeghen P, Soriano E, Peuckert C, Kullander K, Fritzsch B, Nguyen L, Moonen G, Moser T, Malgrange B Ephrin-A5/EphA4 signalling controls specific afferent targeting to cochlear hair cells. Nat Commun, 2013-01-01;4(0):1438. 2013-01-01 [PMID: 23385583] (Bioassay, Mouse)	Bioassay	Mouse
EphA7 Expression Identifies a Unique Neuronal Compartment in the Rat Striatum [PMID: 23348681] (IHC, Mouse)	IHC	Mouse
Szepietowska B, Zhu W, Czyzyk J, Eid T, Sherwin R EphA5-EphrinA5 interactions within the ventromedial hypothalamus influence counterregulatory hormone release and local glutamine/glutamate balance during hypoglycemia. Diabetes, 2012-12-28;62(4):1282-8. 2012-12-28 [PMID: 23274893] (Rat)		Rat
Park , E, Kim , Y, Noh , H, Lee , H, Yoo , S, Park , S EphA/ephrin-A signaling is critically involved in region-specific apoptosis during early brain development. Cell Death Differ, 2012-09-14;20(1):169-80. 2012-09-14 [PMID: 22976838] (Bioassay, Mouse)	Bioassay	Mouse
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)	Bioassay	Human
Genander M, Holmberg J, Frisen J Ephrins negatively regulate cell proliferation in the epidermis and hair follicle. Stem Cells, 2010-07-01;28(7):1196-205. 2010-07-01 [PMID: 20506314] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Afshari FT, Kwok JC, Fawcett JW Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling. J. Neurosci., 2010-03-24;30(12):4246-55. 2010-03-24 [PMID: 20335460] (Bioassay, Rat)	Bioassay	Rat
Torii M, Hashimoto-Torii K, Levitt P, Rakic P Integration of neuronal clones in the radial cortical columns by EphA and ephrin-A signalling. Nature, 2009-09-16;461(7263):524-8. 2009-09-16 [PMID: 19759535] (IHC, Mouse)	IHC	Mouse
Yumoto N, Wakatsuki S, Kurisaki T, Hara Y, Osumi N, Frisen J, Sehara-Fujisawa A Meltrin beta/ADAM19 interacting with EphA4 in developing neural cells participates in formation of the neuromuscular junction. PLoS ONE, 2008-10-02;3(10):e3322. 2008-10-02 [PMID: 18830404] (Bioassay, Mouse)	Bioassay	Mouse
Wykosky J, Palma E, Gibo DM Soluble monomeric EphrinA1 is released from tumor cells and is a functional ligand for the EphA2 receptor. Oncogene, 2008-09-15;27(58):7260-73. 2008-09-15 [PMID: 18794797] (Bioassay, Human)	Bioassay	Human
Lim YS, McLaughlin T, Sung TC, Santiago A, Lee KF, O'Leary DD p75(NTR) mediates ephrin-A reverse signaling required for axon repulsion and mapping. Neuron, 2008-09-11;59(5):746-58. 2008-09-11 [PMID: 18786358] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Fitzgerald M, Buckley A, Lukehurst SS, Dunlop SA, Beazley LD, Rodger J Neurite responses to ephrin-A5 modulated by BDNF: evidence for TrkB-EphA interactions. Biochem. Biophys. Res. Commun., 2008-07-30;374(4):625-30. 2008-07-30 [PMID: 18674513] (Bioassay, Rat)	Bioassay	Rat
Zimmer G, Garcez P, Rudolph J, Niehage R, Weth F, Lent R, Bolz J Ephrin-A5 acts as a repulsive cue for migrating cortical interneurons. Eur. J. Neurosci., 2008-07-01;28(1):62-73. 2008-07-01 [PMID: 18662335] (Bioassay, ICC, Mouse)	Bioassay, ICC	Mouse
Konstantinova I, Nikolova G, Ohara-Imaizumi M, Meda P, Kucera T, Zarbalis K, Wurst W, Nagamatsu S, Lammert E EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets. Cell, 2007-04-20;129(2):359-70. 2007-04-20 [PMID: 17448994] (Bioassay, IHC, Mouse)	Bioassay, IHC	Mouse
Janes PW, Saha N, Barton WA, Kolev MV, Wimmer-Kleikamp SH, Nievergall E, Blobel CP, Himanen JP, Lackmann M, Nikolov DB Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans. Cell, 2005-10-21;123(2):291-304. 2005-10-21 [PMID: 16239146] (Immunoprecipitation, Human)	Immunoprecipitation	Human
Munoz LM, Zayachkivsky A, Kunz RB, Hunt JM, Wang G, Scott SA Ephrin-A5 inhibits growth of embryonic sensory neurons. Dev. Biol., 2005-07-15;283(2):397-408. 2005-07-15 [PMID: 15949793] (ICC, Chicken)	ICC	Chicken
Sharfe N, Freywald A, Toro A, Dadi H, Roifman C Ephrin stimulation modulates T cell chemotaxis. Eur. J. Immunol., 2002-12-01;32(12):3745-55. 2002-12-01 [PMID: 12516569] (Bioassay, Human)	Bioassay	Human
Show All 37 Publications. Collapse Publications.

Array 374-EA

• Ephrin-A5 Antibodies
• Ephrin-A5 ELISA Kit
• Ephrin-A5 Lysate
• Ephrin-A5 Protein
• Ephrin-A5 Proteins and Enzymes