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Recombinant Cotton Rat MIP-2 Protein

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Product Details

Summary
Reactivity CrSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price
Carrier Free
1084-M2-025/CF
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Recombinant Cotton Rat MIP-2 Protein Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 3-15 ng/mL.
Source
E. coli-derived cotton rat CXCL2/GRO beta/MIP-2/CINC-3 protein
Ala29-Asn101
Accession #
N-terminal Sequence
Ala29
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
8.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
1084-M2 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cotton Rat MIP-2 Protein

  • chemokine (C-X-C motif) ligand 2
  • CINC-2a
  • CINC3
  • CINC-3
  • C-X-C Motif Chemokine 2
  • CXCL2
  • GRO beta
  • GRO2 oncogene
  • GRO2
  • GROB
  • Gro-beta
  • Growth-regulated protein beta
  • Macrophage inflammatory protein 2-alpha
  • melanoma growth stimulatory activity beta
  • MGSA beta
  • MGSA-b
  • MGSA-beta
  • MIP2
  • MIP-2
  • MIP2A
  • MIP-2a
  • MIP2-alpha
  • SCYB2

Background

Macrophage inflammatory protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. The protein is produced by a variety of cell types, including intestinal epithelium, macrophages, astrocytes and fibroblasts. Homologs of the mouse MIP-2 have been identified in human, rat and cotton rat. MIP-2 is an ELR-containing member of the alpha (CXC) subfamily of chemokines. The cotton rat MIP-2 gene encodes a 101 amino acid (aa) residue precursor protein with a 28 aa putative signal peptide that is cleaved to generate the 73 aa mature protein. Mature cotton rat MIP-2 has two intrachain disulfide bonds and no potential glycosylation sites. It shares approximately 79% and 85% aa sequence identity with rat CINC-3 and mouse MIP-2, respectively. MIP-2 is a potent neutrophil attractant and activator. MIP-2 binds and activates the chemokine receptor CXCR2. The recombinant cotton rat MIP-2 has been shown to bind and activate the human CXCR2 (1 - 5).

  1. Yoshie, O. et al. (2001) Adv. Immunol. 78:57.
  2. Nakagawa, H. et al. (1994) Biochem. J. 301:545.
  3. Telkamp-Olson, P. et al. (1990) J. Exp. Med. 172:911.
  4. Lee, J. et al. (1995) J. Immunol. 155:2158.
  5. Feng, L. et al. (1995) J. Clin. Invest. 95:1009.

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Publications for CXCL2/GRO beta/MIP-2/CINC-3 (1084-M2)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ELISA (Standard).


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