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RanGAP1 Antibody

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Western Blot: RanGAP1 Antibody [NBP2-20067] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: RanGAP1 Antibody [NBP2-20067] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: RanGAP1 Antibody [NBP2-20067] - Immunohistochemical analysis of paraffin-embedded Trachea, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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RanGAP1 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human RanGAP1. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus membrane; Peripheral membrane protein; Cytoplasmic side; Kinetochore (By similarity)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RANGAP1
Purity
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for RanGAP1 Antibody

  • Fug1
  • KIAA1835Fug1
  • MGC20266
  • Ran GTPase activating protein 1
  • ran GTPase-activating protein 1
  • RanGAP1
  • SD
  • segregation distorter homolog (Drosophila)
  • segregation distorter homolog
  • segregation distortion

Background

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol RANGAP1
Uniprot