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RANBP9 Antibody (SR2288)

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Western Blot: RANBP9 Antibody (SR2288) [NBP3-22340] - Western blot analysis using NBP3-22340 in HeLa cell lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
SR2288
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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RANBP9 Antibody (SR2288) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human RANBP9 (Uniprot #: Q96S59)
Specificity
Detects endogenous levels of total RANBP9
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
RANBP9
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000
Theoretical MW
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for RANBP9 Antibody (SR2288)

  • BPM90
  • BPM-L
  • RAN binding protein 9
  • Ran Binding Protein in the Microtubule organizing center
  • ran binding protein, centrosomal
  • ran-binding protein 9
  • Ran-binding protein M
  • RanBP7
  • RanBP9
  • RanBPM
  • RANBPMnovel centrosomal protein RanBPM

Background

Just as heat shock proteins function as chaperones for exposed hydrophobic patches, importins act as chaperones for exposed basic domains, and it is suggested that this represents a major and general cellular function of importins (1). RanBP9, a novel interacting protein, was localized within the centrosome throughout the cell cycle. Overexpression of RanBP9 produced multiple spots which were colocalized with gamma-tubulin and acted as ectopic microtubule nucleation sites, resulting in a reorganization of microtubule network (2). It has been shown that RanBP9 can induce GTP-Ras association and Erk phosphorylation and elevate serum response element-luciferase (SRE-LUC) expression, indicating that RanBP9 can activate the Ras-Erk-SRE pathway. Also RanBP9 stimulates Ras activation by recruiting Sos (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RANBP9 Antibody (NBP3-22340) (0)

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Secondary Antibodies

 

Isotype Controls

Additional RANBP9 Products

Research Areas for RANBP9 Antibody (NBP3-22340)

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Blogs on RANBP9.

CD68 (Cluster of differentiation 68, GP110, LAMP4, SCARD1)
CD68 belongs to a growing family of hematopoietic mucin-like molecules known as lysosomal/endosomal-associated membrane glycoproteins (LAMPs). Other LAMP family members included leukosialin, stem cell antigen CD34, and GlyCAM-1. CD68 encodes a 110-kD ...  Read full blog post.

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Bioinformatics

Gene Symbol RANBP9