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Puromycin-sensitive aminopeptidase/NPEPPS Antibody

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Western Blot: NPEPPS Antibody [NB100-93421] - (0.3ug/ml) Analysis of Human Brain (Cerebellum) 35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: NPEPPS Antibody [NB100-93421] - Analysis of NPEPPS in Human Brain (Cerebral Cortex) lysate (35ug protein in RIPA buffer) at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Puromycin-sensitive aminopeptidase/NPEPPS Antibody Summary

Immunogen
Peptide with sequence C-KRDAESIHQYLLQRK corresponding to internal region according to NP_006301.2.
Epitope
C-KRDAESIHQYLLQRK
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NPEPPS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.2 - 0.5 ug/ml
Application Notes
WB: Approx. 110 kDa band observed in human brain (cerebral cortex) lysates (calculated MW of 98.5 kDa band according to NP_006301.2).
Publications
Read Publication using NB100-93421.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Puromycin-sensitive aminopeptidase/NPEPPS Antibody

  • AAP-S
  • aminopeptidase puromycin sensitive
  • Cytosol alanyl aminopeptidase
  • EC 3.4.11.2
  • EC 3.4.11.3
  • metalloproteinase MP100
  • MP100
  • MP100EC 3.4.11
  • NPEPPS
  • PSAEC 3.4.11.14
  • puromycin-sensitive aminopeptidase

Background

This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Puromycin-sensitive aminopeptidase/NPEPPS Antibody (NB100-93421)(1)

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Bioinformatics

Gene Symbol NPEPPS
Entrez
Uniprot