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PU.1/Spi-1 Antibody (732322) [DyLight 755]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clone
732322
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 755

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC5870U-100UG
Alexa Fluor 405
IC5870V-100UG
Alexa Fluor 488
IC5870G-100UG
Alexa Fluor 532
FAB5870X
Alexa Fluor 594
IC5870T-100UG
Alexa Fluor 647
IC5870R-100UG
Alexa Fluor 700
IC5870N-100UG
Alexa Fluor 750
IC5870S-100UG
Allophycocyanin
FAB5870A
Biotin
FAB5870B
CoraFluor 1
FAB5870CL1
DyLight 350
FAB5870D
DyLight 405
FAB5870E
DyLight 488
FAB5870K
DyLight 550
FAB5870L
DyLight 594
FAB5870M
DyLight 650
FAB5870W
DyLight 680
FAB5870Y
FITC
FAB5870F
HRP
FAB5870H
Janelia Fluor 525
FAB5870JF525
Janelia Fluor 549
FAB5870I
Janelia Fluor 646
FAB5870J
Janelia Fluor 669
FAB5870JF669
PE
FAB5870P
PerCP
FAB5870C
Unconjugated
MAB5870
mFluor Violet 450 SE
FAB5870MFV450
mFluor Violet 500 SE
FAB5870MFV500
mFluor Violet 610 SE
FAB5870MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB5870
Carrier Free
MAB5870
Carrier Free
MAB5870-SP
Carrier Free
IC5870V-100UG
Carrier Free
IC5870U-100UG
Carrier Free
IC5870T-100UG
Carrier Free
IC5870S-100UG
Carrier Free
IC5870R-100UG
Carrier Free
IC5870N-100UG
Carrier Free
IC5870G-100UG
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PU.1/Spi-1 Antibody (732322) [DyLight 755] Summary

Immunogen
E. coli-derived recombinant human PU.1/Spi-1
Met1-Lys169
Accession # NP_001074016
Specificity
Detects human PU.1/Spi-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Spi-B, recombinant mouse (rm) PU.1/Spi-1, or rmSpi-B is observed. In Western blots, approximately 25% cross-reactivity with rhSpi-B and rmPU.1/Spi-1 is observed.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SPI1
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for PU.1/Spi-1 Antibody (732322) [DyLight 755]

  • hematopoietic transcription factor PU.1
  • OF
  • PU.1
  • SFPI1
  • SFPI131 kDa transforming protein
  • SPI-1 proto-oncogene
  • SPI1
  • Spi-1
  • SPI-A
  • spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein
  • transcription factor PU.1

Background

PU.1/Spi1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SPI1