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PU.1/Spi-1 Antibody (732322) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clone
732322
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

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PU.1/Spi-1 Antibody (732322) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
E. coli-derived recombinant human PU.1/Spi-1
Met1-Lys169
Accession # NP_001074016
Specificity
Detects human PU.1/Spi-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Spi-B, recombinant mouse (rm) PU.1/Spi-1, or rmSpi-B is observed. In Western blots, approximately 25% cross-reactivity with rhSpi-B and rmPU.1/Spi-1 is observed.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SPI1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for PU.1/Spi-1 Antibody (732322) [CoraFluor™ 1]

  • hematopoietic transcription factor PU.1
  • OF
  • PU.1
  • SFPI1
  • SFPI131 kDa transforming protein
  • SPI-1 proto-oncogene
  • SPI1
  • Spi-1
  • SPI-A
  • spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein
  • transcription factor PU.1

Background

PU.1/Spi1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SPI1