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Proprotein Convertase 9/PCSK9 Antibody

 (12 Publications)
 (1 Review)
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Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (5ug/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, ChIP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Proprotein Convertase 9/PCSK9 Antibody Summary

Immunogen
Peptide with sequence CRSRHLAQASQELQ corresponding to C-Terminus according to NP_777596.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PCSK9
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-6 ug/ml
  • Peptide ELISA detection limit 1:16000
  • Western Blot 0.3-1 ug/ml
Application Notes
ICC/IF reactivity reported in (PMID: 30210081), Chip reactivity reported in (PMID: 20427574).
Control
Proprotein Convertase 9/PCSK9 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB300-959 in the following applications:
Publications
Read Publications using
NB300-959 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29593095).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Proprotein Convertase 9/PCSK9 Antibody

  • EC 3.4.21
  • EC 3.4.21.111
  • FH3
  • FH3neural apoptosis regulated convertase 1
  • FHCL3
  • HCHOLA3
  • hypercholesterolemia, autosomal dominant 3
  • LDLCQ1
  • NARC1
  • NARC-1
  • NARC-1convertase subtilisin/kexin type 9 preproprotein
  • NARC1EC 3.4.21.-
  • Neural apoptosis-regulated convertase 1
  • PC9
  • PCSK9
  • Proprotein Convertase 9
  • proprotein convertase subtilisin/kexin type 9
  • Subtilisin/kexin-like protease PC9

Background

This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. The encoded protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. This protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a third form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Proprotein
Convertase 9/PCSK9 An
NB300-959
Proprotein Convertase 9/PCSK9 Antibody
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, ChIP

Publications for Proprotein Convertase 9/PCSK9 Antibody (NB300-959)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Chemotaxis, ICC/IF, WB.


Filter By Application
Chemotaxis
(1)
ICC/IF
(1)
WB
(5)
All Applications
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Human
(2)
Mouse
(1)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB300-959 Applications Species
Tanaka H, Inuzuka K, Iida Y et al. Proprotein Convertase Subtilisin/Kexin Type 9 Is Associated with Degenerating Adipocytes in Abdominal Aortic Aneurysm. J Oleo Sci 2018-09-13 [PMID: 30210081] (ICC/IF, Human) ICC/IF Human
Lebeau P, Platko K, Al-Hashimi AA et al. Loss-of-function PCSK9 mutants evade the unfolded protein response sensor GRP78 and fail to induce endoplasmic reticulum stress when retained J Biol Chem 2018-05-11 [PMID: 29593095] (Mouse) Mouse
Lebeau P, Al-Hashimi A, Sood S et al. Endoplasmic reticulum stress and Ca2+ depletion differentially modulate the sterol-regulatory protein PCSK9 to control lipid metabolism. J. Biol. Chem. 2016-12-01 [PMID: 27909053]
Dube E, Ethier-Chiasson M, Lafond J. Modulation of cholesterol transport by insulin-treated gestational diabetes mellitus in human full-term placenta. Biol Reprod 2013-01-17 [PMID: 23221398]
Brulhart-Meynet MC, Deakin SP, Sgroi A et al. Expression of the hepatic Niemann-Pick C1 like 1 protein gene is sensitive to rosuvastatin treatment of primary human hepatocytes. Pharmacogenet Genomics 2010-07-01 [PMID: 20461026] (WB) WB
Diebold I, Petry A, Hess J et al. The NADPH oxidase subunit NOX4 is a new target gene of the hypoxia-inducible factor-1 Mol Biol Cell. 2010-06-15 [PMID: 20427574] (WB, Chemotaxis) WB, Chemotaxis
Blesa S, Vernia S, Garcia-Garcia AB et al. A new PCSK9 gene promoter variant affects gene expression and causes autosomal dominant hypercholesterolemia. J Clin Endocrinol Metab. 2008-09-01 [PMID: 18559913] (WB)

Details:
WB: human depleted plasma, lymphocytes, and PCSK9 positive control cells (data described but not shown). Albumin and IgG were depleted from the plasma prior to measurement of PCSK9 expression. A WB signal was detected in positive control cells; however, P 		WB
Dewpura T, Raymond A, Hamelin J et al. PCSK9 is phosphorylated by a Golgi casein kinase-like kinase ex vivo and circulates as a phosphoprotein in humans. FEBS J. 2008-07-01 [PMID: 18498363] (WB, Human)

Details:
WB: Media from HepG2 cells, media from PCSK9 overexpressing cells, human plasma (Fig 6). Media was IP'd with a different PCSK9 antibody and immunoprecipitates were detected by WB with the IMG-3786 PCSK9 antibody. Note: The specificity of PCSK9 antibody wa 		WB Human
Lambert G, Ancellin N, Charlton F et al. Plasma PCSK9 concentrations correlate with LDL and total cholesterol in diabetic patients and are decreased by fenofibrate treatment. Clin Chem. 2008-06-01 [PMID: 18436719] (WB)

Details:
WB: Recombinant human PCSK9 (Fig 1B), PCSK9 was detected as a signal band slightly above the 60 kDa marker. ELISA: Human plasma (Figs 1D, 2, 3), The IMG-3786 PSCK9 pAb was used as the capture antibody at 1 ug/well of a 96-well ELISA plate. IP: Human hepta 		WB
Benjannet S, Rhainds D, Hamelin J et al. The proprotein convertase (PC) PCSK9 is inactivated by furin and/or PC5/6A: functional consequences of natural mutations and post-translational modifications. J Biol Chem. 2006-10-13 [PMID: 16912035]

Details:
IP: HEK293 cells transfected with human PCSK9, PCSK9 + Furin, or empty vector (Fig 9A). The PCSK9 IMG-3786 antibody recognized pro-PCSK9, mature PCSK9, and its furin-cleaved form in both cells and media. Note: The specificity of the PCSK9 IMG-3786 antibod
Show All 12 Publications.

Review for Proprotein Convertase 9/PCSK9 Antibody (NB300-959) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB300-959:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Proprotein Convertase 9/PCSK9 NB300-959
Enlarge 		4
reviewed by:
Corey .
 WB Human 04/09/2013
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Summary

ApplicationWestern Blot
SpeciesHuman

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Control Lysate(s)

Proprotein Convertase 9/PCSK9 Overexpression Lysate

Secondary Antibodies

 

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Western Blot Proprotein Convertase 9/PCSK9 NB300-959
Enlarge
 Corey .
04/09/2013
Application: WB
Species: Human
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Bioinformatics

Gene Symbol PCSK9
Entrez
Uniprot