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Human Progesterone R/NR3C3 ELISA Kit (Colorimetric)

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ELISA: Human Progesterone R/NR3C3 ELISA Kit (Colorimetric) [NBP2-68093] - Samples were spiked with high concentrations of Human Progesterone R/NR3C3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Progesterone R/NR3C3 ELISA Kit (Colorimetric) [NBP2-68093] - Samples were spiked with high concentrations of Human Progesterone R/NR3C3 and diluted with Reference Standard & Sample Diluent to produce ...read more
ELISA: Human Progesterone R/NR3C3 ELISA Kit (Colorimetric) [NBP2-68093] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

Order Details

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Human Progesterone R/NR3C3 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human PGR in samples. No significant cross-reactivity or interference between Human PGR and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.33%
Intra-Assay
CV% < 4.95%
Spike Recovery
92-109%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PGR

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Progesterone R/NR3C3 ELISA Kit (Colorimetric)

  • NR3C3
  • NR3C3PRNuclear receptor subfamily 3 group C member 3
  • PGR
  • Progesterone R
  • progesterone receptor
  • ProgesteroneR

Background

The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for Progesterone R/NR3C3 ELISA Kit (NBP2-68093). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in getting antibodies for PR (progesterone receptor) that would work in flow cytometry. Could you recommend some choices?
    • We do not currently have any antibodies directed towards the progesterone receptor that have been validated in flow cytometry, but any of our antibodies that work in ICC should work just fine in flow as they are essentially the same application. Please see this link for our Progesterone Receptor antibodies validated in ICC.

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Product PDFs

  SDS
  Human Progesterone R/NR3C3 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol PGR