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Procalcitonin Antibody

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Western blot shows lysates of human thyroid cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Procalcitonin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8350) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Procalcitonin at approximately 15-16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human thyroid cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Procalcitonin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8350) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF8350-SP
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Procalcitonin Antibody Summary

Immunogen
E. coli-derived recombinant human Procalcitonin
Ala26-Gly117 (Asn57Asp)
Accession # P01258
Specificity
Detects human Procalcitonin in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CALCA
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Procalcitonin Antibody

  • CALC1
  • CALCA
  • Calcitonin 1
  • calcitonin gene-related peptide 1
  • CGRP
  • Katacalcin
  • Procalcitonin

Background

Human Procalcitonin (CALCA and Calcitonin) is 141 aa in length member of the calcitonin family. Precursor protein is cleaved into a propeptide and the following 2 chains: Calcitonin (aa 85-116) and Katacalcin (aa 121-141). Antigen used to develop this antibody is correspondent to the propeptide and Calcitonin part of the precursor molecule. Secreted Calcitonin is known to cause a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Over aa 26-117, human Procalcitonin shares 80% aa identity with mouse Procalcitonin. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CALCA
Uniprot