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PPT1 Antibody (OTI1F10) - Azide and BSA Free

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Western Blot: PPT1 Antibody (OTI1F10) - Azide and BSA Free [NBP2-73588] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-PPT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: ...read more
Immunohistochemistry: PPT1 Antibody (OTI1F10) - Azide and BSA Free [NBP2-73588] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: PPT1 Antibody (OTI1F10) - Azide and BSA Free [NBP2-73588] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPT1.
Immunohistochemistry: PPT1 Antibody (OTI1F10) - Azide and BSA Free [NBP2-73588] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PPT1 Antibody (OTI1F10) - Azide and BSA Free [NBP2-73588] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PPT1 Antibody (OTI1F10) - Azide and BSA Free [NBP2-73588] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PPT1 Antibody (OTI1F10) - Azide and BSA Free [NBP2-73588] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
OTI1F10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

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PPT1 Antibody (OTI1F10) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 100-306 of human PPT1 (NP_000301) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PPT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500
  • Western Blot 1:2000
Theoretical MW
31.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PPT1 Antibody (OTI1F10) - Azide and BSA Free

  • ceroid-palmitoyl-palmitoyl-protein thioesterase 1
  • CLN1EC 3.1.2.22
  • INCL
  • Palmitoyl-protein hydrolase 1
  • palmitoyl-protein thioesterase 1
  • PPT
  • PPT-1

Background

Palmitoyl-protein thioesterase (PPT) is a small glycoprotein that removes palmitate groups from cysteine residues in lipid-modified proteins. PPT is thought to be involved in the catabolism of lipid-modified proteins (Camp et al., 1994 [PubMed 7916016]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PPT1