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PLOD3 293T Cell Transient Overexpression Lysate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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PLOD3 293T Cell Transient Overexpression Lysate Summary

Description
Quality control test: Transient overexpression cell lysate was tested with Anti-PLOD3 antibody by Western Blots. Plasmid: pCMV-PLOD3 full-length
Specificity
PLOD3 293T Cell Transient Overexpression Lysate(Denatured)
Gene
PLOD3

Applications/Dilutions

Dilutions
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

Type
293T Cell Transient Overexpression
Protein State
Denatured

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PLOD3 293T Cell Transient Overexpression Lysate

  • 2-oxoglutarate 5-dioxygenase 3
  • EC 1.14.11.4
  • LH3Lysyl hydroxylase 3
  • lysine hydroxylase 3
  • procollagen-lysine
  • procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3

Background

PLOD3 is encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PLOD3