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Human PLCZ1 ELISA Kit (Colorimetric)

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ELISA: Human PLCZ1 ELISA Kit (Colorimetric) [NBP3-39370] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates and other biological fluids
Standard Curve Range
0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity
0.117 ng/mL (example only; lot dependent)

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Human PLCZ1 ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity
0.117 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PLCZ1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human PLCZ1 ELISA Kit (Colorimetric)

  • MGC149685
  • phosphoinositide phospholipase C-zeta-1
  • phospholipase C, zeta 1
  • PI-phospholipase C zeta 1
  • PLCzeta
  • PLC-zeta-1
  • testis-development protein NYD-SP27
  • testis-development related NYD-SP27

Background

Phospholipases are a group of enzymes that hydrolyze phospholipids into fatty acids and other lipophilic molecules. PLCis subdivided into beta, gamma, delta, epsilon, zeta and eta subtypes, which catalyze the hydrolysis ofphosphatidylinositol 4,5-bisphosphate (PIP2) to inositol 1,4,5-trisphosphate (IP3) and 1,2-diacylglycerol (DAG). IP3and DAG both have important second messenger functions. PLC-beta is primarily activated by Gq/11 proteins andPLC-gamma is activated by phosphorylation in response to a variety of growth factor and immune system signals.Phospholipases are ubiquitously expressed and have diverse biological functions including roles in inflammation, cellgrowth, signaling and death and maintenance of membrane phospholipids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PLCZ1