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Periostin/OSF-2 Antibody (CL12087)

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Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody (CL12087) [NBP3-15128] - Staining of U-138 cells using the Anti-POSTN monoclonal antibody, showing specific staining in vesicles in green. Microtubule- and nuclear probes are visualized in red and blue, respectively.
Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody (CL12087) [NBP3-15128] - Staining of U-138 cells using the Anti-POSTN monoclonal antibody, showing specific staining in vesicles in green. Microtubule- ...read more
Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody (CL12087) [NBP3-15128] - Analysis in human stomach and skeletal muscle tissues using NBP3-15128 antibody. Corresponding POSTN RNA-seq data are presented for the ...read more
Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody (CL12087) [NBP3-15128] - Staining of human astrocytoma shows moderate positivity in tumor cells.
Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody (CL12087) [NBP3-15128] - Staining of human breast cancer shows strong positivity in extracellular matrix.
Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody (CL12087) [NBP3-15128] - Staining of human fallopian tube shows moderate to strong positivity in the extracellular matrix.
Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody (CL12087) [NBP3-15128] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody (CL12087) [NBP3-15128] - Staining of human stomach shows strong positivity in extracellular matrix, as well as moderate positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clone
CL12087
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Periostin/OSF-2 Antibody (CL12087) Summary

Immunogen
This antibody was developed against a synthetic peptide. Actual immunogen sequence is proprietary information.
Predicted Species
Mouse (96%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
POSTN
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Periostin/OSF-2 Antibody (CL12087)

  • Fasciclin I-like
  • MGC119510
  • MGC119511
  • OSF2
  • OSF-2
  • OSF-2osteoblast specific factor 2 (fasciclin I-like)
  • OSF2periodontal ligament-specific periostin
  • Osteoblast-specific factor 2
  • PDLPOSTN
  • periostin isoform thy2
  • periostin isoform thy4
  • periostin isoform thy6
  • periostin isoform thy8
  • Periostin
  • periostin, osteoblast specific factor
  • PNRP11-412K4.1
  • POSTN
  • TRIF52

Background

Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblastspecific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol POSTN