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PDE1B Antibody (OTI6E4) - Azide and BSA Free

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Western Blot: PDE1B Antibody (OTI6E4) - Azide and BSA Free [NBP2-73278] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE1B.
Western Blot: PDE1B Antibody (OTI6E4) - Azide and BSA Free [NBP2-73278] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE1B (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: PDE1B Antibody (OTI6E4) - Azide and BSA Free [NBP2-73278] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE1B.
Immunohistochemistry: PDE1B Antibody (OTI6E4) - Azide and BSA Free [NBP2-73278] - Staining of paraffin-embedded Human Kidney tissue using anti-PDE1B mouse monoclonal antibody.
Flow Cytometry: PDE1B Antibody (OTI6E4) - Azide and BSA Free [NBP2-73278] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PDE1B ...read more
Flow Cytometry: PDE1B Antibody (OTI6E4) - Azide and BSA Free [NBP2-73278] - Analysis of Jurkat cells, using anti-PDE1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI6E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73278AF350
Alexa Fluor 405
NBP2-73278AF405
Alexa Fluor 488
NBP2-73278AF488
Alexa Fluor 532
NBP2-73278AF532
Alexa Fluor 594
NBP2-73278AF594
Alexa Fluor 647
NBP2-73278AF647
Alexa Fluor 700
NBP2-73278AF700
Alexa Fluor 750
NBP2-73278AF750
Allophycocyanin
NBP2-73278APC
Biotin
NBP2-73278B
CoraFluor 1
NBP2-73278CL1
DyLight 350
NBP2-73278UV
DyLight 405
NBP2-73278V
DyLight 488
NBP2-73278G
DyLight 550
NBP2-73278R
DyLight 594
NBP2-73278DL594
DyLight 650
NBP2-73278C
DyLight 680
NBP2-73278FR
DyLight 755
NBP2-73278IR
FITC
NBP2-73278F
HRP
NBP2-73278H
Janelia Fluor 525
NBP2-73278JF525
Janelia Fluor 549
NBP2-73278JF549
Janelia Fluor 585
NBP2-73278JF585
Janelia Fluor 635
NBP2-73278JF635
Janelia Fluor 646
NBP2-73278JF646
Janelia Fluor 669
NBP2-73278JF669
PE
NBP2-73278PE
PerCP
NBP2-73278PCP
Unconjugated
NBP2-01063
mFluor Violet 450 SE
NBP2-73278MFV450
mFluor Violet 500 SE
NBP2-73278MFV500
mFluor Violet 610 SE
NBP2-73278MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-01063
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PDE1B Antibody (OTI6E4) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human PDE1B(NP_000915) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PDE1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
61.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PDE1B Antibody (OTI6E4) - Azide and BSA Free

  • 63 kDa Cam-PDE
  • calcium/calmodulin-dependent 3'-5'-cyclic nucleotide phosphodiesterase 1B
  • Cam-PDE 1B
  • EC 3.1.4
  • EC 3.1.4.17
  • PDE1B
  • PDE1B1calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase
  • PDES1B
  • PDES1Bcalmodulin-stimulated phosphodiesterase PDE1B1
  • phosphodiesterase 1B, calmodulin-dependent
  • phosphodiesterase IB, calmodulin-dependent
  • Phosphodiesterase-1B
  • presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase familyknown as PDE1B

Background

PDE1B, a variant of PDE1, is a Ca2+-calmodulin-stimulated phosphodieterase (PDE) preferentially hydrolyzing cGMP. Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-11) based on their kinetic properties and inhibition to various pharmacological agents. Three different PDE1 genes have been identified (PDE1A, PDE1B, PDE1C). The enzymes of the PDE1A and PDE1B have higher affinity for cGMP than for cAMP, whereas PDE1C has high affinity for both cAMP and cGMP. Calcium-Calmodulin stimulates the activity of each of these enzymes several-fold. The tissue distribution of PDE1B is different from other PDE1 gene family members. In testis PDE1B is expressed uniformly through out the seminiferous epithelium and in the interstistium in contrast to PDE1A and PDE1C that is expressed in elongated to mature spermatids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PDE1B