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Rat PACAP/ADCYAP1 ELISA Kit (Colorimetric)

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ELISA: Rat PACAP/ADCYAP1 ELISA Kit (Colorimetric) [NBP2-78762] - Samples were spiked with high concentrations of Rat PACAP/ADCYAP1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat PACAP/ADCYAP1 ELISA Kit (Colorimetric) [NBP2-78762] - Samples were spiked with high concentrations of Rat PACAP/ADCYAP1 and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Rat PACAP/ADCYAP1 ELISA Kit (Colorimetric) [NBP2-78762] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
7.81 - 500 pg/mL
Sensitivity
4.69 pg/mL

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Rat PACAP/ADCYAP1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat PACAP in samples. No significant cross-reactivity or interference between Rat PACAP and analogues was observed.
Standard Curve Range
7.81 - 500 pg/mL
Sensitivity
4.69 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.45%
Intra-Assay
CV% < 4.20%
Spike Recovery
91-106%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ADCYAP1

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publication using NBP2-78762.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat PACAP/ADCYAP1 ELISA Kit (Colorimetric)

  • ADCYAP1
  • adenylate cyclase activating polypeptide 1 (pituitary)
  • PACAP
  • PACAPMGC126852
  • pituitary adenylate cyclase-activating polypeptide

Background

ADCYAP1 encodes adenylate cyclase activating polypeptide 1. Mediated by adenylate cyclase activating polypeptide 1 receptors, this polypeptide stimulates adenylate cyclase and subsequently increases the cAMP level in target cells. Adenylate cyclase activating polypeptide 1 is not only a hypophysiotropic hormone, but also functions as a neurotransmitter and neuromodulator. In addition, it plays a role in paracrine and autocrine regulation of certain types of cells. This gene encodes three different mature peptides, including two isotypes, a shorter form and a longer form. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for PACAP/ADCYAP1 ELISA Kit (NBP2-78762)(1)


Showing Publication 1 - 1 of 1.
Publication using NBP2-78762 Applications Species
Wang Q, Ma T, Lu Z et al. Xiongzhi Dilong decoction interferes with calcitonin gene-related peptide (CGRP)-induced migraine in rats through the CGRP/iNOS pathway Journal of Traditional Chinese Medical Sciences 2022-06-01

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Product PDFs

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  Rat PACAP/ADCYAP1 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol ADCYAP1